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验证数据展示

  • WB analysis using 31091-1-AP

    WB analysis using 31091-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31091-1-AP (CORO1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 31091-1-AP

    IHC staining of mouse liver using 31091-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31091-1-AP (CORO1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 31091-1-AP

    IHC staining of human ovary cancer using 31091-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31091-1-AP (CORO1B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CORO1B Polyclonal antibody

CORO1B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31091-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Coronin-2, Coronin-1B, CORONIN 2, Coronin 1B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHT-29 cells, MDA-MB-453 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse liver tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31091-1-AP targets CORO1B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CORO1B fusion protein Ag34813 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coronin, actin binding protein, 1B
别名 Coronin-2, Coronin-1B, CORONIN 2, Coronin 1B
计算分子量 498 aa, 54 kDa
观测分子量68 kDa
GenBank蛋白编号BC006449
基因名称 CORO1B
Gene ID (NCBI) 57175
RRIDAB_3669849
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BR76
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CORO1B (Coronin 1B) is an actin-binding protein that controls actin networks at classical lamellipodia (PMID: 32850828). Coro1B localizes to the plasma membrane, regulates lamellipodia formation, and when phosphorylated, it can no longer bind ARP2/3 and inhibit its actin nucleation abilities (PMID: 22619279).

实验方案

Product Specific Protocols
WB protocol for CORO1B antibody 31091-1-APDownload protocol
IHC protocol for CORO1B antibody 31091-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols