验证数据展示
经过测试的应用
| Positive WB detected in | rat heart tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83566-1-RR targets CORIN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag15898 Product name: Recombinant human CORIN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 551-900 aa of BC110451 Sequence: WPEDTDCSQFPEENSDNQTCLMPDEYVEECSPSHFKCRSGQCVLASRRCDGQADCDDDSDEENCGCKERDLWECPSNKQCLKHTVICDGFPDCPDYMDEKNCSFCQDDELECANHACVSRDLWCDGEADCSDSSDEWDCVTLSINVNSSSFLMVHRAATEHHVCADGWQEILSQLACKQMGLGEPSVTKLIQEQEKEPRWLTLHSNWESLNGTTLHELLVNGQSCESRSKISLLCTKQDCGRRPAARMNKRILGGRTSRPGRWPWQCSLQSEPSGHICGCVLIAKKWVLTVAHCFEGRENAAVWKVVLGINNLDHPSVFMQTRFVKTIILHPRYSRAVVDYDISIVELSE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | corin, serine peptidase |
| 别名 | TMPRSS10, serine peptidase, CRN, 240339A10 |
| 计算分子量 | 1042 aa, 117 kDa |
| 观测分子量 | 180 kDa |
| GenBank蛋白编号 | BC110451 |
| 基因名称 | CORIN |
| Gene ID (NCBI) | 10699 |
| RRID | AB_3671181 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q9Y5Q5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CORIN is a transmembrane type II serine protease expressed in cardiomyocytes that converts pro-atrial natriuretic peptide into atrial natriuretic peptide, a cardiac hormone that regulates blood pressure. CORIN can be shed into the circulation through autocleavage and metalloproteinase-mediated hydrolysis processes of the membrane form of the protein. Three different soluble fragments derived from corin molecules, with molecular masses of ~180, ~160 and ~100 kDa were described in literature (PMID: 21288900, 29387201).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CORIN antibody 83566-1-RR | Download protocol |
| WB protocol for CORIN antibody 83566-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
