验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, U-87 MG cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30909-1-AP targets COQ2 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 | COQ2 fusion protein Ag29155 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | coenzyme Q2 homolog, prenyltransferase (yeast) |
| 别名 | CL640, COQ2, COQ2 homolog, FLJ26072, hCOQ2, PHB:polyprenyltransferase |
| 计算分子量 | 371 aa, 40 kDa |
| 观测分子量 | 35 kDa，40 kDa，45 kDa |
| GenBank蛋白编号 | BC008804 |
| 基因名称 | COQ2 |
| Gene ID (NCBI) | 27235 |
| RRID | AB_3669780 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q96H96 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COQ2 (coenzyme Q2, polyprenyltransferase), also known as CL640. It is expected to be located in mitochondrion inner membrane. And the protein is highly expressed in skeletal muscle, adrenal glands and the heart. COQ2 is a redox carrier in the mitochondrial respiratory chain and a lipid-soluble antioxidant. This enzyme, which is part of the coenzyme Q10 pathway, catalyzes the prenylation of parahydroxybenzoate with an all-trans polyprenyl group. Mutations in this gene cause coenzyme Q10 deficiency, a mitochondrial encephalomyopathy, and also COQ2 nephropathy, an inherited form of mitochondriopathy with primary renal involvement. The protein has three isforms, the calculated molecular weight are 35, 40, 45 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for COQ2 antibody 30909-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |