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验证数据展示

  • WB analysis using CL594-66994

    WB analysis using CL594-66994

    HeLa cells were subjected to SDS PAGE followed by western blot with CL594-66994 (COPE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

CoraLite®594-conjugated COPE Monoclonal antibody

COPE Monoclonal Antibody for WB
Cat No. CL594-66994

产品说明书

CloneNo. 2B8E8

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB

别名

Coatomer subunit epsilon, COPE, Epsilon coat protein, epsilon COP, FLJ13241

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy COPE Ready-To-Use IHC Kit. COPE primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66994-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66994 targets COPE in WB applications and shows reactivity with Human samples.

经测试应用 WB Application Description
经测试反应性 Human
免疫原

CatNo: Ag28602

Product name: Recombinant human COPE protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-308 aa of BC017285

Sequence: MAPPAPGPASGGSGEVDELFDVKNAFYIGSYQQCINEAQRVKLSSPERDVERDVFLYRAYLAQRKFGVVLDEIKPSSAPELQAVRMFADYLAHESRRDSIVAELDREMSRSVDVTNTTFLLMAASIYLHDQNPDAALRALHQGDSLECTAMTVQILLKLDRLDLARKELKRMQDLDEDATLTQLATAWVSLATGGEKLQDAYYIFQEMADKCSPTLLLLNGQAACHMAQGRWEAAEGLLQEALDKDSGYPETLVNLIVLSQHLGKPPEVTNRYLSQLKDAHRSHPFIKEYQAKENDFDRLVLQYAPSA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 coatomer protein complex, subunit epsilon
别名 Coatomer subunit epsilon, COPE, Epsilon coat protein, epsilon COP, FLJ13241
计算分子量 34 kDa
观测分子量 32 kDa
GenBank蛋白编号BC017285
基因名称 COPE
Gene ID (NCBI) 11316
RRIDAB_2934754
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDO14579
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CL594 COPE antibody CL594-66994Download protocol
Standard Protocols
Click here to view our Standard Protocols