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验证数据展示

  • WB analysis of mouse liver using 15269-1-AP (same clone as 15269-1-PBS)

    WB analysis of mouse liver using 15269-1-AP (same clone as 15269-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15269-1-AP (COLEC11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15269-1-PBS in a different storage buffer formulation.

  • IF Staining of ATCD5 cells using 15269-1-AP (same clone as 15269-1-PBS)

    IF Staining of ATCD5 cells using 15269-1-AP (same clone as 15269-1-PBS)

    IF result (confocal image) of CL-K1 in ATCD5 cell show Golgi localization from Prof. Philip L. Beales. This data was developed using the same antibody clone with 15269-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 15269-1-AP (same clone as 15269-1-PBS)

    IHC staining of mouse liver using 15269-1-AP (same clone as 15269-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15269-1-AP (COLEC11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15269-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 15269-1-AP (same clone as 15269-1-PBS)

    IHC staining of mouse liver using 15269-1-AP (same clone as 15269-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15269-1-AP (COLEC11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15269-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 15269-1-AP (same clone as 15269-1-PBS)

    IP experiment of mouse liver using 15269-1-AP (same clone as 15269-1-PBS)

    IP result of anti-COLEC11 (IP:15269-1-AP, 4ug; Detection:15269-1-AP 1:500) with mouse liver tissue lysate 6400ug. This data was developed using the same antibody clone with 15269-1-PBS in a different storage buffer formulation.

COLEC11 Polyclonal antibody, PBS Only

COLEC11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 15269-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15269-1-PBS targets COLEC11 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7374

Product name: Recombinant human COLEC11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 18-271 aa of BC000078

Sequence: SLLPSGHPQPAGDDACSVQILVPGLKGDAGEKGDKGAPGRPGRVGPTGEKGDMGDKGQKGSVGRHGKIGPIGSKGEKGDSGDIGPPGPNGEPGLPCECSQLRKAIGEMDNQVSQLTSELKFIKNAVAGVRETESKIYLLVKEEKRYADAQLSCQGRGGTLSMPKDEAANGLMAAYLAQAGLARVFIGINDLEKEGAFVYSDHSPMRTFNKWRSGEPNNAYDEEDCVEMVASGGWNDVACHTTMYFMCEFDKENM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 collectin sub-family member 11
别名
计算分子量 29 kDa
观测分子量 34 kDa
GenBank蛋白编号BC000078
基因名称 COLEC11
Gene ID (NCBI) 78989
RRIDAB_10732940
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BWP8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

COLEC11, also named as CL-K1 and Collectin-11, belongs to the COLEC10/COLEC11 family. It is a lectin that binds to various sugars: fucose > mannose. It does not bind to glucose, N-acetylglucosamine and N-acetylgalactosamine. COLEC11 binds to LPS. COLEC11 and MASP1 are two genes in the lectin complement pathway, they are mutated in 3MC syndrome, implicating this diverse inflammation-chemotaxis cascade in the etiology of human developmental disorders. COLEC11 serves as a guidance cue for neural crest cell migration.(PMID:21258343)

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