验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, human plasma |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | ATCD5 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15269-1-AP targets COLEC11 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag7374 Product name: Recombinant human COLEC11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 18-271 aa of BC000078 Sequence: SLLPSGHPQPAGDDACSVQILVPGLKGDAGEKGDKGAPGRPGRVGPTGEKGDMGDKGQKGSVGRHGKIGPIGSKGEKGDSGDIGPPGPNGEPGLPCECSQLRKAIGEMDNQVSQLTSELKFIKNAVAGVRETESKIYLLVKEEKRYADAQLSCQGRGGTLSMPKDEAANGLMAAYLAQAGLARVFIGINDLEKEGAFVYSDHSPMRTFNKWRSGEPNNAYDEEDCVEMVASGGWNDVACHTTMYFMCEFDKENM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | collectin sub-family member 11 |
| 别名 | Collectin-11, UNQ596/PRO1182, Collectin 11, CL-K1, CLK1 |
| 计算分子量 | 29 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC000078 |
| 基因名称 | COLEC11 |
| Gene ID (NCBI) | 78989 |
| RRID | AB_10732940 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BWP8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COLEC11, also named as CL-K1 and Collectin-11, belongs to the COLEC10/COLEC11 family. It is a lectin that binds to various sugars: fucose > mannose. It does not bind to glucose, N-acetylglucosamine and N-acetylgalactosamine. COLEC11 binds to LPS. COLEC11 and MASP1 are two genes in the lectin complement pathway, they are mutated in 3MC syndrome, implicating this diverse inflammation-chemotaxis cascade in the etiology of human developmental disorders. COLEC11 serves as a guidance cue for neural crest cell migration.(PMID:21258343)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for COLEC11 antibody 15269-1-AP | Download protocol |
| IP protocol for COLEC11 antibody 15269-1-AP | Download protocol |
| WB protocol for COLEC11 antibody 15269-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Genet Mutations in lectin complement pathway genes COLEC11 and MASP1 cause 3MC syndrome. | ||
Front Immunol Development of a Quantitative Assay for the Characterization of Human Collectin-11 (CL-11, CL-K1). | ||
J Immunol Heterocomplex Formation between MBL/Ficolin/CL-11-Associated Serine Protease-1 and -3 and MBL/Ficolin/CL-11-Associated Protein-1. | ||
J Innate Immun Collectin-11/MASP Complex Formation Triggers Activation of the Lectin Complement Pathway - The Fifth Lectin Pathway Initiation Complex. | ||
Clin Proteomics iTRAQ plasma proteomics analysis for candidate biomarkers of type 2 incipient diabetic nephropathy. |
