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验证数据展示

  • WB analysis using 12738-1-AP

    WB analysis using 12738-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12738-1-AP (Collagen Type XXV antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 12738-1-AP

    IF Staining of HeLa using 12738-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Collagen Type XXV antibody (12738-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of Neuro-2a using 12738-1-AP

    IF Staining of Neuro-2a using 12738-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using Collagen Type XXV antibody (12738-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Collagen Type XXV Polyclonal antibody

Collagen Type XXV Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 12738-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

COL25, COL25A1, Alzheimer disease amyloid-associated protein, CLAC-P, COL 25A1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells, Neuro-2a cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12738-1-AP targets Collagen Type XXV in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3634

Product name: Recombinant human COL25A1 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 165-497 aa of BC036669

Sequence: GPLGPPGQKGSIGAPGIPGMNGQKGEPGLPGAVGQNGIPGPKGEPGEQGEKGDAGENGPKGDTGEKGDPGSSAAGIKGEPGESGRPGQKGEPGLPGLPGLPGIKGEPGFIGPQGEPGLPGLPGTKGERGEAGPPGRGERGEPGAPGPKGKQGESGTRGPKGSKGDRGEKGDSGAQGPRGPPGQKGDQGATEIIDYNGNLHEALQRITTLTVTGPPGPPGPQGLQGTKGEQGSPGIPGMDGEQGLKGSKGDMGDPGSQWNERRKRRFWNAGSTGSFYHRPTRPTRSPWPTWPHGTSWTSWTKGSIWLRRKARIPGYRWSYGTPWPCRSQRRKR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 collagen, type XXV, alpha 1
别名 COL25, COL25A1, Alzheimer disease amyloid-associated protein, CLAC-P, COL 25A1
计算分子量65 kDa
观测分子量55 kDa
GenBank蛋白编号BC036669
基因名称 COL25A1
Gene ID (NCBI) 84570
RRIDAB_2291945
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BXS0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Collagen alpha-1(XXV) chain is a protein that in humans is encoded by the COL25A1 gene. COL25A1 is a brain-specific membrane-bound collagen. Proteolytic processing releases CLAC, a soluble form of COL25A1 containing the extracellular collagen domains that associates with senile plaques in Alzheimer disease brains.(PMID: 11927537)

实验方案

Product Specific Protocols
IF protocol for Collagen Type XXV antibody 12738-1-APDownload protocol
WB protocol for Collagen Type XXV antibody 12738-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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