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验证数据展示

  • WB analysis using 30045-1-AP (same clone as 30045-1-PBS)

    WB analysis using 30045-1-AP (same clone as 30045-1-PBS)

    Various lysates were subjected to Tris-Acetate gel system followed by western blot with 30045-1-AP (COL12A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30045-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 30045-1-AP (same clone as 30045-1-PBS)

    IF Staining of U2OS using 30045-1-AP (same clone as 30045-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using COL12A1 antibody (30045-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 30045-1-PBS in a different storage buffer formulation.

Collagen Type XII Polyclonal antibody, PBS Only

Collagen Type XII Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 30045-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

COL12, COL12A1, COL12A1L, Collagen alpha 1(XII) chain, Collagen alpha-1(XII) chain

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30045-1-PBS targets Collagen Type XII in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag32602

Product name: Recombinant human COL12A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 768-892 aa of NM_004370

Sequence: VTTPPNQRRRTLENLIPDTKYEVSVIPEYFSGPGTPLTGNAATEEVRGNPRDLRVSDPTTSTMKLSWSGAPGKVKQYLVTYTPVAGGETQEVTVRGDTTNTVLQGLKEGTQYALSVTALYASGAG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 collagen, type XII, alpha 1
别名 COL12, COL12A1, COL12A1L, Collagen alpha 1(XII) chain, Collagen alpha-1(XII) chain
计算分子量333kd
观测分子量310-333 kDa
GenBank蛋白编号NM_004370
基因名称 COL12A1
Gene ID (NCBI) 1303
RRIDAB_3742325
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99715
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

COL12A1 (Collagen alpha-1(XII) chain), also known as COL12A1L. It is predicted to be located in the ECM. Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix. The molecular weight of COL12A1 is 333 kDa.

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