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验证数据展示

  • WB analysis of HeLa using 67218-1-Ig (same clone as 67218-1-PBS)

    WB analysis of HeLa using 67218-1-Ig (same clone as 67218-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67218-1-Ig (COIL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67218-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67218-1-Ig (same clone as 67218-1-PBS)

    IF Staining of HepG2 using 67218-1-Ig (same clone as 67218-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COIL antibody (67218-1-Ig, Clone: 2G12B8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67218-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67218-1-Ig (same clone as 67218-1-PBS)

    FC experiment of HeLa using 67218-1-Ig (same clone as 67218-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human COIL (67218-1-Ig, Clone:2G12B8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67218-1-PBS in a different storage buffer formulation.

COIL Monoclonal antibody, PBS Only

COIL Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 67218-1-PBS

产品说明书

CloneNo. 2G12B8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

Coilin, 2G12B8, CLN80, p80 coilin, p80-coilin

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67218-1-PBS targets COIL in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 COIL fusion protein Ag17171 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 coilin
别名 Coilin, 2G12B8, CLN80, p80 coilin, p80-coilin
计算分子量 576 aa, 63 kDa
观测分子量 80 kDa
GenBank蛋白编号BC010385
基因名称 COIL
Gene ID (NCBI) 8161
RRIDAB_2882509
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP38432
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C