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COIL Monoclonal antibody

COIL Monoclonal Antibody for FC, IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC, IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G12B8

Cat No : 67218-1-Ig

Print datasheet

Synonyms

CLN80, COIL, coilin, p80, p80 coilin

验证数据展示

  • WB analysis of HeLa using 67218-1-Ig

    WB analysis of HeLa using 67218-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67218-1-Ig (COIL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 67218-1-Ig

    IF Staining of HepG2 using 67218-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COIL antibody (67218-1-Ig, Clone: 2G12B8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of HeLa using 67218-1-Ig

    FC experiment of HeLa using 67218-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human COIL (67218-1-Ig, Clone:2G12B8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67218-1-Ig targets COIL in WB, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, IF/ICC, WB, ELISA
Tested Reactivity Human
Immunogen COIL fusion protein Ag17171 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name coilin
Synonyms CLN80, COIL, coilin, p80, p80 coilin
Calculated Molecular Weight 576 aa, 63 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC010385
Gene Symbol COIL
Gene ID (NCBI) 8161
RRIDAB_2882509
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP38432
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for COIL antibody 67218-1-IgDownload protocol
IF protocol for COIL antibody 67218-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols