×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 87612-1-RR (same clone as 87612-1-PBS)

    WB analysis using 87612-1-RR (same clone as 87612-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87612-1-RR (CNTN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87612-1-PBS in a different storage buffer formulation.

  • IP experiment of rat brain using 87612-1-RR (same clone as 87612-1-PBS)

    IP experiment of rat brain using 87612-1-RR (same clone as 87612-1-PBS)

    IP result of anti-CNTN1 (IP:87612-1-RR, 4ug; Detection:87612-1-RR 1:10000) with rat brain tissue lysate 1640 ug. This data was developed using the same antibody clone with 87612-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 87612-1-RR (same clone as 87612-1-PBS)

    IP experiment of mouse brain using 87612-1-RR (same clone as 87612-1-PBS)

    IP result of anti-CNTN1 (IP:87612-1-RR, 4ug; Detection:87612-1-RR 1:10000) with mouse brain tissue lysate 1680 ug. This data was developed using the same antibody clone with 87612-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP03227-1

    Sandwich ELISA sample test of MP03227-1

    The mean CNTN1 concentration was determined to be 429.57 ng/mL in A549 cell extract based on a 1.20 mg/mL extract load and 191.70 ng/mL in HT-29 cell extract based on a 1.10 mg/mL extract load.

  • Sandwich ELISA standard curve of MP03227-1

    Sandwich ELISA standard curve of MP03227-1

    Sandwich ELISA standard curve of MP03227-1, Human CNTN1 Recombinant Matched Antibody Pair - PBS only. 87612-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg6555. 87612-1-PBS was HRP conjugated as the detection antibody. Range: 7.81-500 ng/mL.

  • IF Staining of mouse brain using 87612-1-RR (same clone as 87612-1-PBS)

    IF Staining of mouse brain using 87612-1-RR (same clone as 87612-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CNTN1 antibody (87612-1-RR, Clone: 252906D4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87612-1-PBS in a different storage buffer formulation.

  • Affinity of 87612-1-RR

    Affinity of 87612-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87612-1-RR against Human CNTN1 were performed. The affinity constant is 1.72 nM.

CNTN1 Recombinant monoclonal antibody, PBS Only (Detector)

CNTN1 Uni-rAb® Recombinant Antibody for WB, IF-P, IP, Sandwich ELISA, Indirect ELISA
Cat No. 87612-1-PBS

产品说明书

CloneNo. 252906D4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, IP, Sandwich ELISA, Indirect ELISA

别名

contactin 1, Contactin-1, F3, Glycoprotein gp135, GP135

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

87612-1-PBS targets CNTN1 as part of a matched antibody pair:

MP03227-1: 87612-2-PBS capture and 87612-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF-P, IP, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg6555

Product name: Recombinant Human CNTN1 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 21-993 aa of NM_001843.3

Sequence: EFTWYRRYGHGVSEEDKGFGPIFEEQPINTIYPEESLEGKVSLNCRARASPFPVYKWRMNNGDVDLTSDRYSMVGGNLVINNPDKQKDAGIYYCLASNNYGMVRSTEATLSFGYLDPFPPEERPEVRVKEGKGMVLLCDPPYHFPDDLSYRWLLNEFPVFITMDKRRFVSQTNGNLYIANVEASDKGNYSCFVSSPSITKSVFSKFIPLIPIPERTTKPYPADIVVQFKDVYALMGQNVTLECFALGNPVPDIRWRKVLEPMPSTAEISTSGAVLKIFNIQLEDEGIYECEAENIRGKDKHQARIYVQAFPEWVEHINDTEVDIGSDLYWPCVATGKPIPTIRWLKNGYAYHKGELRLYDVTFENAGMYQCIAENTYGAIYANAELKILALAPTFEMNPMKKKILAAKGGRVIIECKPKAAPKPKFSWSKGTEWLVNSSRILIWEDGSLEINNITRNDGGIYTCFAENNRGKANSTGTLVITDPTRIILAPINADITVGENATMQCAASFDPALDLTFVWSFNGYVIDFNKENIHYQRNFMLDSNGELLIRNAQLKHAGRYTCTAQTIVDNSSASADLVVRGPPGPPGGLRIEDIRATSVALTWSRGSDNHSPISKYTIQTKTILSDDWKDAKTDPPIIEGNMEAARAVDLIPWMEYEFRVVATNTLGRGEPSIPSNRIKTDGAAPNVAPSDVGGGGGRNRELTITWAPLSREYHYGNNFGYIVAFKPFDGEEWKKVTVTNPDTGRYVHKDETMSPSTAFQVKVKAFNNKGDGPYSLVAVINSAQDAPSEAPTEVGVKVLSSSEISVHWEHVLEKIVESYQIRYWAAHDKEEAANRVQVTSQEYSARLENLLPDTQYFIEVGACNSAGCGPPSDMIEAFTKKAPPSQPPRIISSVRSGSRYIITWDHVVALSNESTVTGYKVLYRPDGQHDGKLYSTHKHSIEVPIPRDGEYVVEVRAHSDGGDGVVSQVKIS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 contactin 1
别名 contactin 1, Contactin-1, F3, Glycoprotein gp135, GP135
计算分子量113 kDa
观测分子量135 kDa
GenBank蛋白编号NM_001843.3
基因名称 CNTN1
Gene ID (NCBI) 1272
RRIDAB_3745649
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ12860
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Contactin-1 (CNTN1), also known as Glycoprotein gp135 or Neural cell surface protein F3, is a neuronal cell adhesion molecule of the immunoglobulin superfamily (PMID: 3049624). It consists of six Ig domains, four fibronectin-like motifs, and a glycosylphosphatidylinositol (GPI)-moiety. Expressed by the central and peripheral neurons, CNTN1 contributes to the formation and function of neuronal connections. It plays a role in axon extension and formation of septate-like junctions between axons and myelinating glial cells (PMID: 23724143). In addition, acting as a ligand for Notch, CNTN1 initiates a NOTCH/DTX1 signaling pathway that promotes oligodendrocyte maturation and myelination (PMID: 14567914).

||
New chat

Able™

正在加载，请稍候...