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CNTF Polyclonal antibody

CNTF Polyclonal Antibody for IF/ICC, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC, IHC,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 27342-1-AP

Print datasheet

Synonyms

ciliary neurotrophic factor, CNTF, HCNTF

验证数据展示

  • IHC staining of human cerebellum using 27342-1-AP

    IHC staining of human cerebellum using 27342-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 27342-1-AP (CNTF Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human cerebellum using 27342-1-AP

    IHC staining of human cerebellum using 27342-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 27342-1-AP (CNTF Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of PC-12 using 27342-1-AP

    IF Staining of PC-12 using 27342-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using 27342-1-AP (CNTF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inhuman cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-12 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

The immunogen of 27342-1-AP is CNTF Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC,ELISA
Cited ApplicationsIF, Cell treatment
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen CNTF fusion protein Ag26410 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ciliary neurotrophic factor
Synonyms ciliary neurotrophic factor, CNTF, HCNTF
Calculated Molecular Weight 200 aa, 23 kDa
GenBank Accession NumberBC074964
Gene Symbol CNTF
Gene ID (NCBI) 1270
RRIDAB_2880848
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP26441
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for CNTF antibody 27342-1-APDownload protocol
IF protocol for CNTF antibody 27342-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanCell treatment

Transl Psychiatry

Chronic cortisol differentially impacts stem cell-derived astrocytes from major depressive disorder patients.

Authors - Kelly J Heard
View Article
mouseIF

bioRxiv

Lipid nanoparticle-mediated delivery of mRNA into the mouse and human retina and other ocular tissues

Authors - Cheri Z Chambers
View Article