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验证数据展示

  • WB analysis of mouse pancreas using 14635-1-AP (same clone as 14635-1-PBS)

    WB analysis of mouse pancreas using 14635-1-AP (same clone as 14635-1-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14635-1-AP (CNPY2,MSAP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.

  • WB analysis using 14635-1-AP (same clone as 14635-1-PBS)

    WB analysis using 14635-1-AP (same clone as 14635-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14635-1-AP (CNPY2, MSAP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 14635-1-AP (same clone as 14635-1-PBS)

    IP experiment of HepG2 using 14635-1-AP (same clone as 14635-1-PBS)

    IP result of anti-CNPY2, MSAP (IP:14635-1-AP, 3ug; Detection:14635-1-AP 1:500) with HepG2 cells lysate 600ug. This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 14635-1-AP (same clone as 14635-1-PBS)

    IHC staining of human liver using 14635-1-AP (same clone as 14635-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14635-1-AP (CNPY2, MSAP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 14635-1-AP (same clone as 14635-1-PBS)

    IF Staining of HepG2 using 14635-1-AP (same clone as 14635-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 14635-1-AP (CNPY2,MSAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 14635-1-AP (same clone as 14635-1-PBS)

    FC experiment of HepG2 using 14635-1-AP (same clone as 14635-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CNPY2, MSAP (14635-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 14635-1-AP (same clone as 14635-1-PBS)

    WB analysis of HepG2 using 14635-1-AP (same clone as 14635-1-PBS)

    WB result of CNPY2, MSAP antibody (14635-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPY2, MSAP transfected HepG2 cells. This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 14635-1-AP (same clone as 14635-1-PBS)

    IHC staining of human liver using 14635-1-AP (same clone as 14635-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14635-1-AP (CNPY2, MSAP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 14635-1-AP (same clone as 14635-1-PBS)

    IF Staining of HepG2 using 14635-1-AP (same clone as 14635-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CNPY2, MSAP antibody (14635-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14635-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CNPY2/MSAP Polyclonal antibody, PBS Only

CNPY2/MSAP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 14635-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

CNPY2, CNPY2, MSAP, CNPY2,MSAP, canopy 2 homolog (zebrafish), HP10390

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

14635-1-PBS targets CNPY2/MSAP in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6214

Product name: Recombinant human CNPY2,MSAP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-182 aa of BC065015

Sequence: MKGWGWLALLLGALLGTAWARRSQDLHCGACRALVDELEWEIAQVDPKKTIQMGSFRINPDGSQSVVEVPYARSEAHLTELLEEICDRMKEYGEQIDPSTHRKNYVRVVGRNGESSELDLQGIRIDSDISGTLKFACESIVEEYEDELIEFFSREADNVKDKLCSKRTDLCDHALHISHDEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 canopy 2 homolog (zebrafish)
别名 CNPY2, CNPY2, MSAP, CNPY2,MSAP, canopy 2 homolog (zebrafish), HP10390
计算分子量 21 kDa
观测分子量 17-21 kDa
GenBank蛋白编号BC065015
基因名称 CNPY2
Gene ID (NCBI) 10330
RRIDAB_2082621
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2B0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth. It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 can stabilize MRLC and thus bring about an increase in neurite outgrowth. (PMID: 12826659 ) This antibody is specific to CNPY2.

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