验证数据展示

WB analysis of mouse pancreas using 14635-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14635-1-AP (CNPY2,MSAP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 14635-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14635-1-AP (CNPY2, MSAP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 14635-1-AP
IP result of anti-CNPY2, MSAP (IP:14635-1-AP, 3ug; Detection:14635-1-AP 1:500) with HepG2 cells lysate 600ug.
IHC staining of human liver using 14635-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14635-1-AP (CNPY2, MSAP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).
IF Staining of HepG2 using 14635-1-AP
Immunofluorescent analysis of HepG2 cells using 14635-1-AP (CNPY2,MSAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 14635-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CNPY2, MSAP (14635-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.
WB analysis of HepG2 using 14635-1-AP
WB result of CNPY2, MSAP antibody (14635-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPY2, MSAP transfected HepG2 cells.
IHC staining of human liver using 14635-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14635-1-AP (CNPY2, MSAP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).
IF Staining of HepG2 using 14635-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CNPY2, MSAP antibody (14635-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Featured Product
KD/KO验证

CNPY2/MSAP Polyclonal antibody

CNPY2/MSAP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 14635-1-AP

产品说明书

引用文献(17)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

别名

CNPY2, CNPY2, MSAP, CNPY2,MSAP, canopy 2 homolog (zebrafish), HP10390

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (17)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HepG2 cells, mouse pancreas tissue, human liver tissue, mouse liver tissue, rat liver tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human liver tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 5 publications below
WB	See 15 publications below
IHC	See 5 publications below
IF	See 4 publications below

产品信息

14635-1-AP targets CNPY2/MSAP in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat
免疫原	CatNo: Ag6214 Product name: Recombinant human CNPY2,MSAP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-182 aa of BC065015 Sequence: MKGWGWLALLLGALLGTAWARRSQDLHCGACRALVDELEWEIAQVDPKKTIQMGSFRINPDGSQSVVEVPYARSEAHLTELLEEICDRMKEYGEQIDPSTHRKNYVRVVGRNGESSELDLQGIRIDSDISGTLKFACESIVEEYEDELIEFFSREADNVKDKLCSKRTDLCDHALHISHDEL 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	canopy 2 homolog (zebrafish)
别名	CNPY2, CNPY2, MSAP, CNPY2,MSAP, canopy 2 homolog (zebrafish), HP10390
计算分子量	21 kDa
观测分子量	17-21 kDa
GenBank蛋白编号	BC065015
基因名称	CNPY2
Gene ID (NCBI)	10330
RRID	AB_2082621
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q9Y2B0
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth. It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 can stabilize MRLC and thus bring about an increase in neurite outgrowth. (PMID: 12826659 ) This antibody is specific to CNPY2.

实验方案

Product Specific Protocols
IF protocol for CNPY2/MSAP antibody 14635-1-AP	Download protocol
IHC protocol for CNPY2/MSAP antibody 14635-1-AP	Download protocol
IP protocol for CNPY2/MSAP antibody 14635-1-AP	Download protocol
WB protocol for CNPY2/MSAP antibody 14635-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse,human	WB	Nat Struct Mol Biol CNPY2 is a key initiator of the PERK-CHOP pathway of the unfolded protein response. Authors - Feng Hong KO Validated View Article
mouse	IHC	Cancers (Basel) Canopy Homolog 2 as a Novel Molecular Target in Hepatocarcinogenesis. Authors - Anna Kakehashi View Article
human	WB,IF	Apoptosis Angiotensin-(1-9) prevents angiotensin II-induced endothelial apoptosis through CNPY2/PERK pathway Authors - Chun-Ling Guo KD Validated View Article
rat	WB	J Cell Mol Med PMID: 31625681 Authors - Lina Zhao View Article
mouse	WB	J Biol Chem Fibroblast growth factor-21 (FGF21) regulates low-density lipoprotein receptor (LDLR) levels in cells via the E3-ubiquitin ligase Mylip/Idol and the Canopy2 (Cnpy2)/Mylip-interacting saposin-like protein (Msap). Authors - Do Hai Thi HT KD Validated View Article
rat	WB	J Biol Chem Reciprocal regulation of Very low-density lipoprotein receptors (VLDLRs) in neurons by Brain-derived neurotrophic factor (BDNF) and Reelin: Involvement of the E3 ligase Mylip/Idol. Authors - Do Hai Thi HT View Article

验证数据展示

WB analysis of mouse pancreas using 14635-1-AP

WB analysis using 14635-1-AP

IP experiment of HepG2 using 14635-1-AP

IHC staining of human liver using 14635-1-AP

IF Staining of HepG2 using 14635-1-AP

FC experiment of HepG2 using 14635-1-AP

WB analysis of HepG2 using 14635-1-AP

IHC staining of human liver using 14635-1-AP

IF Staining of HepG2 using 14635-1-AP

CNPY2/MSAP Polyclonal antibody

CNPY2/MSAP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

CNPY2 is a key initiator of the PERK-CHOP pathway of the unfolded protein response.

Canopy Homolog 2 as a Novel Molecular Target in Hepatocarcinogenesis.

Angiotensin-(1-9) prevents angiotensin II-induced endothelial apoptosis through CNPY2/PERK pathway

PMID: 31625681

Fibroblast growth factor-21 (FGF21) regulates low-density lipoprotein receptor (LDLR) levels in cells via the E3-ubiquitin ligase Mylip/Idol and the Canopy2 (Cnpy2)/Mylip-interacting saposin-like protein (Msap).

Reciprocal regulation of Very low-density lipoprotein receptors (VLDLRs) in neurons by Brain-derived neurotrophic factor (BDNF) and Reelin: Involvement of the E3 ligase Mylip/Idol.