• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

CNPY2, MSAP Polyclonal antibody

CNPY2, MSAP Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 14635-1-AP

Print datasheet

Synonyms

canopy 2 homolog (zebrafish), CNPY2, CNPY2,MSAP, HP10390, MSAP, Protein canopy homolog 2, TMEM4, Transmembrane protein 4, ZSIG9

验证数据展示

  • WB analysis of mouse pancreas using 14635-1-AP

    WB analysis of mouse pancreas using 14635-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14635-1-AP (CNPY2,MSAP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis using 14635-1-AP

    WB analysis using 14635-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14635-1-AP (CNPY2, MSAP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 14635-1-AP

    IP experiment of HepG2 using 14635-1-AP

    IP result of anti-CNPY2, MSAP (IP:14635-1-AP, 3ug; Detection:14635-1-AP 1:500) with HepG2 cells lysate 600ug.

  • IHC staining of human liver using 14635-1-AP

    IHC staining of human liver using 14635-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14635-1-AP (CNPY2, MSAP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  • IF Staining of HepG2 using 14635-1-AP

    IF Staining of HepG2 using 14635-1-AP

    Immunofluorescent analysis of HepG2 cells using 14635-1-AP (CNPY2,MSAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 14635-1-AP

    FC experiment of HepG2 using 14635-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CNPY2, MSAP (14635-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of HepG2 using 14635-1-AP

    WB analysis of HepG2 using 14635-1-AP

    WB result of CNPY2, MSAP antibody (14635-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPY2, MSAP transfected HepG2 cells.

  • IHC staining of human liver using 14635-1-AP

    IHC staining of human liver using 14635-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14635-1-AP (CNPY2, MSAP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  • IF Staining of HepG2 using 14635-1-AP

    IF Staining of HepG2 using 14635-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CNPY2, MSAP antibody (14635-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHepG2 cells, mouse pancreas tissue, human liver tissue, mouse liver tissue, rat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14635-1-AP targets CNPY2, MSAP in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen CNPY2, MSAP fusion protein Ag6214 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name canopy 2 homolog (zebrafish)
Synonyms canopy 2 homolog (zebrafish), CNPY2, CNPY2,MSAP, HP10390, MSAP, Protein canopy homolog 2, TMEM4, Transmembrane protein 4, ZSIG9
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 17-21 kDa
GenBank Accession NumberBC065015
Gene Symbol CNPY2
Gene ID (NCBI) 10330
RRIDAB_2082621
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2B0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth. It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 can stabilize MRLC and thus bring about an increase in neurite outgrowth. (PMID: 12826659 ) This antibody is specific to CNPY2.

实验方案

Product Specific Protocols
WB protocol for CNPY2, MSAP antibody 14635-1-APDownload protocol
IHC protocol for CNPY2, MSAP antibody 14635-1-APDownload protocol
IF protocol for CNPY2, MSAP antibody 14635-1-APDownload protocol
IP protocol for CNPY2, MSAP antibody 14635-1-APDownload protocol
FC protocol for CNPY2, MSAP antibody 14635-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Nat Struct Mol Biol

CNPY2 is a key initiator of the PERK-CHOP pathway of the unfolded protein response.

Authors - Feng Hong
  • KO Validated
View Article
mouseIHC

Cancers (Basel)

Canopy Homolog 2 as a Novel Molecular Target in Hepatocarcinogenesis.

Authors - Anna Kakehashi
View Article
humanWB,IF

Apoptosis

Angiotensin-(1-9) prevents angiotensin II-induced endothelial apoptosis through CNPY2/PERK pathway

Authors - Chun-Ling Guo
  • KD Validated
View Article
ratWB

J Cell Mol Med

PMID: 31625681

Authors - Lina Zhao
View Article
mouseWB

J Biol Chem

Fibroblast growth factor-21 (FGF21) regulates low-density lipoprotein receptor (LDLR) levels in cells via the E3-ubiquitin ligase Mylip/Idol and the Canopy2 (Cnpy2)/Mylip-interacting saposin-like protein (Msap).

Authors - Do Hai Thi HT
  • KD Validated
View Article
ratWB

J Biol Chem

Reciprocal regulation of Very low-density lipoprotein receptors (VLDLRs) in neurons by Brain-derived neurotrophic factor (BDNF) and Reelin: Involvement of the E3 ligase Mylip/Idol.

Authors - Do Hai Thi HT
View Article