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Species Reactivity
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验证数据展示

  • WB analysis using 86091-1-RR

    WB analysis using 86091-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86091-1-RR (CNOT8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 86091-1-RR

    Affinity and Kinetic Characterization of 86091-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86091-1-RR against Human CNOT8 were performed. The affinity constant is 0.726 nM.

CNOT8 Recombinant antibody

CNOT8 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 86091-1-RR

产品说明书

CloneNo. 250482E2

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, ELISA

别名

CAF1, CAF1 like protein, Caf1b, CAF1-like protein, CAF2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, HL-60 cells, U-251 cells, rat testis tissue
Planning an IHC experiment? We recommend our IHCeasy CNOT8 Ready-To-Use IHC Kit. CNOT8 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86091-1-RR targets CNOT8 in WB, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, rat
免疫原 CNOT8 fusion protein Ag1201 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CCR4-NOT transcription complex, subunit 8
别名 CAF1, CAF1 like protein, Caf1b, CAF1-like protein, CAF2
计算分子量 34 kDa
观测分子量34 kDa
GenBank蛋白编号BC017366
基因名称 CNOT8
Gene ID (NCBI) 9337
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UFF9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCR4-NOT transcription complex subunit 8(CNOT8), is a ubiquitous transcription factor, which required for a diverse set of processes. The CCR4-NOT complex is a global regulator of RNA polymerase II, and functions as general transcription regulation complex. It consisted part by CCR4, NOT1 to NOT5, and CAF1.

实验方案

Product Specific Protocols
WB protocol for CNOT8 antibody 86091-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols