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验证数据展示

  • IF Staining of human colon cancer using CL488-67798

    IF Staining of human colon cancer using CL488-67798

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 CNOT4 antibody (CL488-67798, Clone: 4H5H3 ) at dilution of 1:200.

  • IF Staining of HepG2 using CL488-67798

    IF Staining of HepG2 using CL488-67798

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CNOT4 antibody (CL488-67798, Clone: 4H5H3 ) at dilution of 1:800.

CoraLite® Plus 488-conjugated CNOT4 Monoclonal antibody

CNOT4 Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL488-67798

产品说明书

CloneNo. 4H5H3

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

IF/ICC, IF-P

别名

E3 ubiquitin-protein ligase CNOT4, CLONE243, CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4, CCR4 associated factor 4

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF-P detected inhuman colon cancer tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CNOT4 Ready-To-Use IHC Kit. CNOT4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67798-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67798 targets CNOT4 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag29964

Product name: Recombinant human CNOT4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 273-572 aa of BC035590

Sequence: IDKPSDSLSIGNGDNSQQISNSDTPSPPPGLSKSNPVIPISSSNHSARSPFEGAVTESQSLFSDIFRHPNPIPSGLPPFPSSPQTSSDWPTAPEPQSLFTSETIPVSSSTDWQAAFGFGSSKQPEDDLGFDPFDVTRKALADLIEKELSVQDQPSLSPTSLQNSSSHTTTAKGPGSGFLHPAAATNANSLNSTFSVLPQRFPQFQQHRAVYNSFSFPGQAARYPWMAFPRNSIMHLNHTANPTSNSNFLDLNLPPQHNTGLGGIPVAGEEEVKVSTMPLSTSSHSLQQGQQPTSLHTTVA

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 CCR4-NOT transcription complex, subunit 4
别名 E3 ubiquitin-protein ligase CNOT4, CLONE243, CCR4-NOT transcription complex subunit 4, CCR4-associated factor 4, CCR4 associated factor 4
计算分子量 575 aa, 64 kDa
GenBank蛋白编号BC035590
基因名称 CNOT4
Gene ID (NCBI) 4850
RRIDAB_2919550
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDO95628
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CNOT4, also known as CCR4-NOT transcription complex subunit 4 , has E3 ubiquitin ligase activity, and promotes ubiquitination and degradation of target proteins. CNOT4 is involved in activation of the JAK/STAT pathway. CNOT4 exist many isoforms and the range of its molecular weight from 48 to 84 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CNOT4 antibody CL488-67798Download protocol
Standard Protocols
Click here to view our Standard Protocols
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