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验证数据展示

  • IF Staining of HepG2 using CL488-15938

    IF Staining of HepG2 using CL488-15938

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CNOT10 antibody (CL488-15938) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-15938

    FC experiment of HepG2 using CL488-15938

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Cnot10 Polyclonal Antibody (CL488-15938)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite® Plus 488-conjugated CNOT10 Polyclonal antibody

CNOT10 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-15938

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

CCR4-NOT transcription complex subunit 10

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CNOT10 Ready-To-Use IHC Kit. CNOT10 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15938-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15938 targets CNOT10 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7654

Product name: Recombinant human CNOT10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 568-717 aa of BC002928

Sequence: NPENVTDVSLGISSNEQDQGSDKGENEAMESSGKRAPQCYPSSVNSARTVMLFNLGSAYCLRSEYDKARKCLHQAASMIHPKEVPPEAILLAVYLELQNGNTQLALQIIKRNQLLPAVKTHSEVRKKPVFQPVHPIQPIQMSAFTTVQRK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CCR4-NOT transcription complex, subunit 10
别名 CCR4-NOT transcription complex subunit 10
计算分子量 82 kDa
观测分子量 82 kDa
GenBank蛋白编号BC002928
基因名称 CNOT10
Gene ID (NCBI) 25904
RRIDAB_3672642
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H9A5
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CNOT10, also named as CCR4-NOT transcription complex subunit 10, is a 744 amino acid protein, which belongs to the CNOT10 family. CNOT10 lacalizes in the cytoplasm and nucleus. CNOT10 is a component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CNOT10 antibody CL488-15938Download protocol
IF protocol for CL Plus 488 CNOT10 antibody CL488-15938Download protocol
Standard Protocols
Click here to view our Standard Protocols
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