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CNDP2 Polyclonal antibody

CNDP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14925-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

HEL-S-13, Glutamate carboxypeptidase-like protein 1, EC:3.4.13.18, Cytosolic non-specific dipeptidase, CPGL

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHepG2 cells, LNCaP cells, mouse kidney tissue, rat kidney rat kidney
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CNDP2 Ready-To-Use IHC Kit. CNDP2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14925-1-AP targets CNDP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, zebrafish
免疫原 CNDP2 fusion protein Ag6684 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CNDP dipeptidase 2 (metallopeptidase M20 family)
别名 HEL-S-13, Glutamate carboxypeptidase-like protein 1, EC:3.4.13.18, Cytosolic non-specific dipeptidase, CPGL
计算分子量 53 kDa
观测分子量 44-53 kDa
GenBank蛋白编号BC001375
基因名称 CNDP2
Gene ID (NCBI) 55748
RRIDAB_2081682
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96KP4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Carnosine dipeptidase II (CNDP2) catalyzes the peptide bond hydrolysis in dipeptides, displaying a non-redundant activity toward threonyl dipeptides. It has high dipeptidase activity toward cysteinyl glycine, an intermediate metabolite in glutathione metabolism (PubMed:19346245, PubMed:12473676). Metabolizes N-lactoyl-amino acids, both through hydrolysis to form lactic acid and amino acids, as well as through their formation by reverse proteolysis (PubMed:25964343). CNDP2 has 2 isoforms with a molecular weight of 53 and 44 kDa.
The isoform 1 is ubiquitously expressed with higher levels in the kidney and liver (at protein level) and expressed in peripheral blood leukocytes (PubMed:12473676). It is also expressed in gastric mucosa and down-regulated in gastric cancer mucosal tissues (at the protein level) (PubMed:24395568). Isoform 2 is broadly expressed in fetal tissues and expressed in the adult liver and placenta. (PMID: 17121880).

实验方案

Product Specific Protocols
WB protocol for CNDP2 antibody 14925-1-APDownload protocol
IHC protocol for CNDP2 antibody 14925-1-APDownload protocol
IF protocol for CNDP2 antibody 14925-1-APDownload protocol
IP protocol for CNDP2 antibody 14925-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IHC

Acta Neuropathol

The proteome of granulovacuolar degeneration and neurofibrillary tangles in Alzheimer's disease.

Authors - David C Hondius
mouseWB

Cell Chem Biol

Family-wide Annotation of Enzymatic Pathways by Parallel In Vivo Metabolomics.

Authors - Joon T Kim
  • KO Validated
humanWB,IHC

Mol Med

Underexpressed CNDP2 participates in gastric cancer growth inhibition through activating the MAPK signaling pathway.

Authors - Zhenwei Zhang
humanWB,IF

BMC Cancer

The relationship between pepsinogen C and gastric carcinogenesis: a transgene and population study

Authors - Ying E
humanWB

PLoS One

Quantitative proteomics approach to screening of potential diagnostic and therapeutic targets for laryngeal carcinoma.

Authors - Li Li
humanWB,IHC

BMC Gastroenterol

Up-regulation of CNDP2 facilitates the proliferation of colon cancer.

Authors - Conglong Xue
  • KD Validated
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