验证数据展示
经过测试的应用
| Positive WB detected in | J774A.1 cells, K-562 cells, Raji cells, RAW 264.7 cells |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12851-1-AP targets CMIP in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag3475 Product name: Recombinant human CMIP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 314-620 aa of BC038113 Sequence: DDWRPSLASLLQPIPFPKEALAHEKFTKELKYVIQRFAEDPRQEVHSCLLSVRAGKDGWFQLYSPGGVACDDDGELFASMVHILMGSCYKTKKFLLSLAENKLGPCMLLALRGNQTMVEILCLMLEYNIIDNNDTQLQIISTLESTDVGKRMYEQLCDRQRELKELQRKGGPTRLTLPSKSTDADLARLLSSGSFGNLENLSLAFTNVTSACAEHLIKLPSLKQLNLWSTQFGDAGLRLLSEHLTMLQVLNLCETPVTDAGLLALSSMKSLCSLNMNSTKLSADTYEDLKAKLPNLKEVDVRYTEAW 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | c-Maf-inducing protein |
| 别名 | c Maf inducing protein, c Mip, C-Maf-inducing protein, c-Mip, Tc Mip |
| 计算分子量 | 86 kDa |
| 观测分子量 | 86 kDa |
| GenBank蛋白编号 | BC038113 |
| 基因名称 | CMIP |
| Gene ID (NCBI) | 80790 |
| RRID | AB_2081366 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IY22 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The gene of CMIP was isolated from one patient of minimal change nephrotic syndrome. The gene encodes two isoforms: CMIP and truncated CMIP. The predicted CMIP and truncated CMIP proteins contain 739 and 679 amino acids and have calculated molecular masses of 85 and 75 kD, respectively. The two isoforms have different distribution and expression pattern.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CMIP antibody 12851-1-AP | Download protocol |
| IP protocol for CMIP antibody 12851-1-AP | Download protocol |
| WB protocol for CMIP antibody 12851-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab FALCON systematically interrogates free fatty acid biology and identifies a novel mediator of lipotoxicity | ||
Biochem Pharmacol Magnesium isoglycyrrhizinate ameliorates fructose-induced podocyte apoptosis through downregulation of miR-193a to increase WT1. | ||
Mol Med Rep c‑Maf inducing protein inhibits cofilin‑1 activity and alters podocyte cytoskeleton organization. | ||
Cell Signal Inhibition of nephrin activation by c-mip through Csk-Cbp-Fyn axis plays a critical role in Angiotensin II-induced podocyte damage.
| ||
Eur J Hum Genet CMIP as a novel candidate gene for neurodevelopmental and neuropsychiatric disorders. |
