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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85399-4-RR (same clone as 85399-4-PBS)

    WB analysis using 85399-4-RR (same clone as 85399-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85399-4-RR (CLYBL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85399-4-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 85399-4-RR (same clone as 85399-4-PBS)

    WB analysis of HSC-T6 using 85399-4-RR (same clone as 85399-4-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 85399-4-RR (CLYBL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85399-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85399-4-RR

    Affinity and Kinetic Characterization of 85399-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85399-4-RR against Human CLYBL were performed. The affinity constant is below 1 pM.

CLYBL Recombinant antibody, PBS Only

CLYBL Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85399-4-PBS

产品说明书

CloneNo. 242785F10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

(3S)-malyl-CoA thioesterase, Beta-methylmalate synthase, Citramalyl-CoA lyase, mitochondrial, citrate lyase beta like, Citrate lyase beta-like

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CLYBL Ready-To-Use IHC Kit. CLYBL primary antibody included.

产品信息

85399-4-PBS targets CLYBL in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CLYBL fusion protein Ag11283 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 citrate lyase beta like
别名 (3S)-malyl-CoA thioesterase, Beta-methylmalate synthase, Citramalyl-CoA lyase, mitochondrial, citrate lyase beta like, Citrate lyase beta-like
计算分子量 340 aa, 37 kDa
观测分子量 30-40 kDa
GenBank蛋白编号BC034360
基因名称 CLYBL
Gene ID (NCBI) 171425
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8N0X4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CLYBL, also named CLB, is a Mitochondrial citramalyl-CoA lyase indirectly involved in vitamin B12 metabolism (PubMed:29056341). It is a human mitochondrial enzyme of unknown function that is found in multiple eukaryotic taxa and conserved to bacteria. It acts as a beta-methylmalate synthase in vitro, by mediating the conversion of glyoxylate and propionyl-CoA to beta-methylmalate. There are two isoforms with MW 31-33 kDa and 35-37 kDa.