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验证数据展示

  • WB analysis using 85399-4-RR

    WB analysis using 85399-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85399-4-RR (CLYBL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 85399-4-RR

    WB analysis of HSC-T6 using 85399-4-RR

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 85399-4-RR (CLYBL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85399-4-RR

    Affinity and Kinetic Characterization of 85399-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85399-4-RR against Human CLYBL were performed. The affinity constant is below 1 pM.

CLYBL Recombinant antibody

CLYBL Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85399-4-RR

产品说明书

CloneNo. 242785F10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

(3S)-malyl-CoA thioesterase, Beta-methylmalate synthase, Citramalyl-CoA lyase, mitochondrial, citrate lyase beta like, Citrate lyase beta-like

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, HSC-T6 cells, THP-1 cells
Planning an IHC experiment? We recommend our IHCeasy CLYBL Ready-To-Use IHC Kit. CLYBL primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85399-4-RR targets CLYBL in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CLYBL fusion protein Ag11283 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 citrate lyase beta like
别名 (3S)-malyl-CoA thioesterase, Beta-methylmalate synthase, Citramalyl-CoA lyase, mitochondrial, citrate lyase beta like, Citrate lyase beta-like
计算分子量 340 aa, 37 kDa
观测分子量 30-40 kDa
GenBank蛋白编号BC034360
基因名称 CLYBL
Gene ID (NCBI) 171425
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8N0X4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CLYBL, also named CLB, is a Mitochondrial citramalyl-CoA lyase indirectly involved in vitamin B12 metabolism (PubMed:29056341). It is a human mitochondrial enzyme of unknown function that is found in multiple eukaryotic taxa and conserved to bacteria. It acts as a beta-methylmalate synthase in vitro, by mediating the conversion of glyoxylate and propionyl-CoA to beta-methylmalate. There are two isoforms with MW 31-33 kDa and 35-37 kDa.

实验方案

Product Specific Protocols
WB protocol for CLYBL antibody 85399-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols