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验证数据展示

  • IF Staining of HepG2 using CL594-66487

    IF Staining of HepG2 using CL594-66487

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66487 (CLTC antibody) at dilution of 1:100.

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  • KD/KO验证

CoraLite®594-conjugated CLTC Monoclonal antibody

CLTC Monoclonal Antibody for IF/ICC
Cat No. CL594-66487

产品说明书

CloneNo. 1B3D7

宿主/亚型

Mouse / IgG1

种属反应性

Human, Pig, Mouse, Rat

应用

IF/ICC

别名

CHC, CHC17, Clathrin heavy chain 1, clathrin, heavy chain (Hc), CLH 17, CLH17, CLTC, CLTCL2, Hc, KIAA0034

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CLTC Ready-To-Use IHC Kit. CLTC primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66487-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66487 targets CLTC in IF/ICC applications and shows reactivity with Human, Pig, Mouse, Rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Pig, Mouse, Rat
免疫原

CatNo: Ag25040

Product name: Recombinant human CLTC protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-351 aa of BC054489

Sequence: MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVVIIDMNDPSNPIRRPISADSAIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGESQPVKMFDRHSSLAGCQIINYRTDAKQKWLLLTGISAQQNRVVGAMQLYSVDRKVSQPIEGHAASFAQFKMEGNAEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITNVLQNPDLALRM

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 clathrin, heavy chain (Hc)
别名 CHC, CHC17, Clathrin heavy chain 1, clathrin, heavy chain (Hc), CLH 17, CLH17, CLTC, CLTCL2, Hc, KIAA0034
计算分子量 192 kDa
观测分子量 170 kDa
GenBank蛋白编号BC054489
基因名称 CLTC
Gene ID (NCBI) 1213
RRIDAB_2883592
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ00610
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (CLTC) (160-190 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The heavy chain provides the structural backbone of the clathrin lattice. (PMID: 9133677)

实验方案

Product Specific Protocols
IF protocol for CL594 CLTC antibody CL594-66487Download protocol
Standard Protocols
Click here to view our Standard Protocols
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