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验证数据展示

  • IF Staining of HeLa using CL488-26523

    IF Staining of HeLa using CL488-26523

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 CLTC antibody (CL488-26523) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-26523

    FC experiment of HepG2 using CL488-26523

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 CLTC Polyclonal Antibody (CL488-26523)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated CLTC Polyclonal antibody

CLTC Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-26523

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

IF/ICC, FC (Intra)

别名

CLH 17, clathrin, heavy chain (Hc), Clathrin heavy chain 1, Clathrin heavy chain, CHC17

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CLTC Ready-To-Use IHC Kit. CLTC primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 26523-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-26523 targets CLTC in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原 CLTC fusion protein Ag25041 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 clathrin, heavy chain (Hc)
别名 CLH 17, clathrin, heavy chain (Hc), Clathrin heavy chain 1, Clathrin heavy chain, CHC17
计算分子量 192 kDa
观测分子量 180 kDa
GenBank蛋白编号BC054489
基因名称 CLTC
Gene ID (NCBI) 1213
RRIDAB_3672799
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ00610
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (CLTC) (160-190 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The heavy chain provides the structural backbone of the clathrin lattice. (PMID: 9133677)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CLTC antibody CL488-26523Download protocol
Standard Protocols
Click here to view our Standard Protocols