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验证数据展示

  • WB analysis of pig brain using 66270-1-Ig (same clone as 66270-1-PBS)

    WB analysis of pig brain using 66270-1-Ig (same clone as 66270-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66270-1-Ig (CLTB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66270-1-Ig (same clone as 66270-1-PBS)

    IHC staining of human liver using 66270-1-Ig (same clone as 66270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66270-1-Ig (CLTB Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CLTB antibody included
  • IHC staining of human liver using 66270-1-Ig (same clone as 66270-1-PBS)

    IHC staining of human liver using 66270-1-Ig (same clone as 66270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66270-1-Ig (CLTB Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CLTB antibody included
  • IF Staining of HeLa using 66270-1-Ig (same clone as 66270-1-PBS)

    IF Staining of HeLa using 66270-1-Ig (same clone as 66270-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66270-1-Ig(CLTB antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66270-1-PBS in a different storage buffer formulation.

CLTB Monoclonal antibody, PBS Only

CLTB Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66270-1-PBS

产品说明书

CloneNo. 1F9C2

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Clathrin light chain B, clathrin, light chain (Lcb), CLTB, LCB

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CLTB Ready-To-Use IHC Kit. CLTB primary antibody included.

产品信息

66270-1-PBS targets CLTB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag24347

Product name: Recombinant human CLTB protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-211 aa of BC006457

Sequence: MADDFGFFSSSESGAPEAAEEDPAAAFLAQQESEIAGIENDEGFGAPAGSHAAPAQPGPTSGAGSEDMGTTVNGDVFQEANGPADGYAAIAQADRLTQEPESIRKWREEQRKRLQELDAASKVTEQEWREKAKKDLEEWNQRQSEQVEKNKINNRASEEAFVKESKEETPGTEWEKVAQLCDFNPKSSKQCKDVSRLRSVLMSLKQTPLSR

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 clathrin, light chain (Lcb)
别名 Clathrin light chain B, clathrin, light chain (Lcb), CLTB, LCB
计算分子量 23 kDa
观测分子量 32-38 kDa
GenBank蛋白编号BC006457
基因名称 CLTB
Gene ID (NCBI) 1212
RRIDAB_2881655
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP09497
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)

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