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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated CLTA Polyclonal antibody

CLTA Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat No : CL647-10852

Print datasheet

Synonyms

Clathrin light chain A, clathrin, light chain (Lca), CLTA, LCA

验证数据展示

  • IF Staining of HepG2 using CL647-10852

    IF Staining of HepG2 using CL647-10852

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 647 CLTA antibody (CL647-10852) at dilution of 1:200.

经过测试的应用

Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CLTA Ready-To-Use IHC Kit. CLTA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10852-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-10852 targets CLTA in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity human, mouse, rat
Immunogen CLTA fusion protein Ag1299 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name clathrin, light chain (Lca)
Synonyms Clathrin light chain A, clathrin, light chain (Lca), CLTA, LCA
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC019287
Gene Symbol CLTA
Gene ID (NCBI) 1211
RRIDAB_2920215
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09496
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (180 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The light chain subunits are thought to regulate the formation or disassembly of clathrin coats. (PMID: 2445759; 8374173; 3563513)

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 CLTA antibody CL647-10852Download protocol
Standard Protocols
Click here to view our Standard Protocols