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验证数据展示

  • IHC staining of mouse brain using 15337-1-AP (same clone as 15337-1-PBS)

    IHC staining of mouse brain using 15337-1-AP (same clone as 15337-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15337-1-AP (CLPTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15337-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 15337-1-AP (same clone as 15337-1-PBS)

    IHC staining of mouse brain using 15337-1-AP (same clone as 15337-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15337-1-AP (CLPTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15337-1-PBS in a different storage buffer formulation.

  • WB analysis using 15337-1-AP (same clone as 15337-1-PBS)

    WB analysis using 15337-1-AP (same clone as 15337-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15337-1-AP (CLPTM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15337-1-PBS in a different storage buffer formulation.

  • IP experiment of A549 using 15337-1-AP (same clone as 15337-1-PBS)

    IP experiment of A549 using 15337-1-AP (same clone as 15337-1-PBS)

    IP result of anti-CLPTM1 (IP:15337-1-AP, 4ug; Detection:15337-1-AP 1:300) with A549 cells lysate 1050 ug. This data was developed using the same antibody clone with 15337-1-PBS in a different storage buffer formulation.

CLPTM1 Polyclonal antibody, PBS Only

CLPTM1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15337-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

Putative lipid scramblase CLPTM1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15337-1-PBS targets CLPTM1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7538

Product name: Recombinant human CLPTM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC004865

Sequence: MAAAQEADGARSAVVAAGGGSSGQVTSNGSIGRDPPAETQPQNPPAQPAPNAWQVIKGVLFRIFIIWAISSWFRRGPAPQDQAGPGGAPRVASRNLFPKDTLMNLHVYISEHEHFTDFNATSALFWEQHDLVYGDWTSGENSDGCYEHFAELDIPQSVQQNGSIYIHVYFTKSGFHPDPRQKALYRRLATVHMSRMINKYKRRRFQKTKNLLTGETEADPEMIKRAEDYGPVEVISHWHPNITINIVDDHTPWVKGSVPPPLDQYVKFDAVSGDYYPIIYFNDYWNLQKDYYPINESLASLPLRVSFCPLSLWRWQLYAAQSTKSPWNFLGDELYEQSDEEQDSVKVALLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cleft lip and palate associated transmembrane protein 1
别名 Putative lipid scramblase CLPTM1
计算分子量 62 kDa
观测分子量 65-75 kDa
GenBank蛋白编号BC004865
基因名称 CLPTM1
Gene ID (NCBI) 1209
RRIDAB_3669204
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO96005
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cleft lip and palate transmembrane protein 1 (CLPTM1), a multipass transmembrane protein, was identified due to the association of a chromosomal translocation with a cleft lip and palate (PMID: 9828125). CLPTM1 regulates epileptic seizures by modulating GABAAR-mediated inhibitory synaptic transmission (PMID: 36519412). Highly expressed CLPTM1L may contribute to a poor prognosis and increase invasion, proliferation and migration of oral squamous cell carcinoma (PMID: 34586883).

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