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验证数据展示

  • IHC staining of mouse brain using 15337-1-AP

    IHC staining of mouse brain using 15337-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15337-1-AP (CLPTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 15337-1-AP

    IHC staining of mouse brain using 15337-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15337-1-AP (CLPTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 15337-1-AP

    WB analysis using 15337-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15337-1-AP (CLPTM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of A549 using 15337-1-AP

    IP experiment of A549 using 15337-1-AP

    IP result of anti-CLPTM1 (IP:15337-1-AP, 4ug; Detection:15337-1-AP 1:300) with A549 cells lysate 1050 ug.

CLPTM1 Polyclonal antibody

CLPTM1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 15337-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

Putative lipid scramblase CLPTM1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inA549 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15337-1-AP targets CLPTM1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7538

Product name: Recombinant human CLPTM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC004865

Sequence: MAAAQEADGARSAVVAAGGGSSGQVTSNGSIGRDPPAETQPQNPPAQPAPNAWQVIKGVLFRIFIIWAISSWFRRGPAPQDQAGPGGAPRVASRNLFPKDTLMNLHVYISEHEHFTDFNATSALFWEQHDLVYGDWTSGENSDGCYEHFAELDIPQSVQQNGSIYIHVYFTKSGFHPDPRQKALYRRLATVHMSRMINKYKRRRFQKTKNLLTGETEADPEMIKRAEDYGPVEVISHWHPNITINIVDDHTPWVKGSVPPPLDQYVKFDAVSGDYYPIIYFNDYWNLQKDYYPINESLASLPLRVSFCPLSLWRWQLYAAQSTKSPWNFLGDELYEQSDEEQDSVKVALLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cleft lip and palate associated transmembrane protein 1
别名 Putative lipid scramblase CLPTM1
计算分子量 62 kDa
观测分子量 65-75 kDa
GenBank蛋白编号BC004865
基因名称 CLPTM1
Gene ID (NCBI) 1209
RRIDAB_3669204
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO96005
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cleft lip and palate transmembrane protein 1 (CLPTM1), a multipass transmembrane protein, was identified due to the association of a chromosomal translocation with a cleft lip and palate (PMID: 9828125). CLPTM1 regulates epileptic seizures by modulating GABAAR-mediated inhibitory synaptic transmission (PMID: 36519412). Highly expressed CLPTM1L may contribute to a poor prognosis and increase invasion, proliferation and migration of oral squamous cell carcinoma (PMID: 34586883).

实验方案

Product Specific Protocols
WB protocol for CLPTM1 antibody 15337-1-APDownload protocol
IHC protocol for CLPTM1 antibody 15337-1-APDownload protocol
IP protocol for CLPTM1 antibody 15337-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols