验证数据展示
经过测试的应用
| Positive WB detected in | human heart tissue, pig heart tissue |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66271-1-Ig targets CLPP in WB, IHC, IF, ELISA applications and shows reactivity with human, pig samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, pig |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag8420 Product name: Recombinant human CLPP protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-277 aa of BC002956 Sequence: MWPGILVGGARVASCRYPALGPRLAAHFPAQRPPQRTLQNGLALQRCLHATATRALPLIPIVVEQTGRGERAYDIYSRLLRERIVCVMGPIDDSVASLVIAQLLFLQSESNKKPIHMYINSPGGVVTAGLAIYDTMQYILNPICTWCVGQAASMGSLLLAAGTPGMRHSLPNSRIMIHQPSGGARGQATDIAIQAEEIMKLKKQLYNIYAKHTKQSLQVIESAMERDRYMSPMEAQEFGILDKVLVHPPQDGEDEPTLVQKEPVEAAPAAEPVPAST 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG3 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli) |
| 别名 | 2E1D9, ATP-dependent Clp protease proteolytic subunit, mitochondrial, EC:3.4.21.92, Endopeptidase Clp |
| 计算分子量 | 30 kDa |
| 观测分子量 | 26 kDa, 30 kDa |
| GenBank蛋白编号 | BC002956 |
| 基因名称 | CLPP |
| Gene ID (NCBI) | 8192 |
| RRID | AB_2881656 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q16740 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CLPP is the putative ATP-dependent Clp protease proteolytic subunit and also named as endopeptidase Clp. It belongs to the peptidase S14 family. Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. It may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. This protein has a transit peptide of 56 amino acid.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CLPP antibody 66271-1-Ig | Download protocol |
| WB protocol for CLPP antibody 66271-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cells Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA. | ||
PeerJ Mitochondrial unfolded protein response gene CLPP changes mitochondrial dynamics and affects mitochondrial function.
| ||
NeuroImmune Pharm Ther Resveratrol and its analogs suppress HIV replication, oxidative stress, and inflammation in macrophages | ||
Front Pharmacol BAM8-22 targets spinal MrgC receptors to modulate UPRmt activity in the mechanism of bone cancer pain |
