×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis of HEK-293 using 18094-1-AP (same clone as 18094-1-PBS)

    WB analysis of HEK-293 using 18094-1-AP (same clone as 18094-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18094-1-AP (CLOCK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18094-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 18094-1-AP (same clone as 18094-1-PBS)

    IP experiment of HeLa using 18094-1-AP (same clone as 18094-1-PBS)

    IP result of anti-CLOCK (IP:18094-1-AP, 4ug; Detection:18094-1-AP 1:300) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 18094-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 18094-1-AP (same clone as 18094-1-PBS)

    IF Staining of HeLa using 18094-1-AP (same clone as 18094-1-PBS)

    Immunofluorescent analysis of HeLa cells using 18094-1-AP (CLOCK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18094-1-PBS in a different storage buffer formulation.

  • WB analysis using 18094-1-AP (same clone as 18094-1-PBS)

    WB analysis using 18094-1-AP (same clone as 18094-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18094-1-AP (CLOCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18094-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 18094-1-AP (same clone as 18094-1-PBS)

    IHC staining of human liver using 18094-1-AP (same clone as 18094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18094-1-AP (CLOCK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18094-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CLOCK antibody included
  • IHC staining of human liver using 18094-1-AP (same clone as 18094-1-PBS)

    IHC staining of human liver using 18094-1-AP (same clone as 18094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18094-1-AP (CLOCK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18094-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CLOCK antibody included
  • Featured Product
  • KD/KO Validated

CLOCK Polyclonal antibody, PBS Only

CLOCK Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 18094-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy CLOCK Ready-To-Use IHC Kit. CLOCK primary antibody included.

产品信息

18094-1-PBS targets CLOCK in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag12826

Product name: Recombinant human CLOCK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC041878

Sequence: MLFTVSCSKMSSIVDRDDSSIFDGLVEEDDKDKAKRVSRNKSEKKRRDQFNVLIKELGSMLPGNARKMDKSTVLQKSIDFLRKHKEITAQSDASEIRQDWKPTFISNEEFTQLMLEALDGFFLAIMTDGSIIYVSESVTSLLEHLPSDLVDQSIFNFIPEGEHSEVYKILSTHLLESDSLTPEYLKSKNQLEFCCHMLRGTIDPKEPSTYEYVKFIGNFKSLNSVSSSAHNGFEGTIQRTHRPSYEDRVCFVATVRLATPQFIKEMCTVEEPNEEFTSRHSLEWKFLFLDHRAPPIIGYLPFEVLGTSGYDYYHVDDLENLAKCHEHLMQYGKGKSCYYRFLTKGQQWIWL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 clock homolog (mouse)
别名
计算分子量 846 aa, 95 kDa
观测分子量 95-110 kDa
GenBank蛋白编号BC041878
基因名称 CLOCK
Gene ID (NCBI) 9575
RRIDAB_2878497
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15516
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Circadian locomoter output cycles protein kaput (CLOCK), also named as BHLHE8 or KIAA0334, is a 846 amino acid protein, which contains one bHLH domain, one PAC domain, and two PAS domains. CLOCK localizes in the nucleus and cytoplasm. CLOCK) is expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. ARNTL/2-CLOCK heterodimers activate E-box element (5'-CACGTG-3') transcription of a number of proteins of the circadian clock, such as PER1 and PER2. The calculated molecular weight of CLOCK is 95kDa, we detected a 95-110 kDa protein by western blot. Our result is similar to the result of reseach paper (PMID: 30683868).

||
New chat

Able™

正在加载，请稍候...