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CLOCK Polyclonal antibody

CLOCK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18094-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

bHLHe8, CLOCK, clock homolog (mouse), hCLOCK, KAT13D, KIAA0334

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, PC-3 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CLOCK Ready-To-Use IHC Kit. CLOCK primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18094-1-AP targets CLOCK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF, IHC
经测试反应性 human
文献引用反应性human, mouse
免疫原 CLOCK fusion protein Ag12826 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 clock homolog (mouse)
别名 bHLHe8, CLOCK, clock homolog (mouse), hCLOCK, KAT13D, KIAA0334
计算分子量 846 aa, 95 kDa
观测分子量 95-110 kDa
GenBank蛋白编号BC041878
基因名称 CLOCK
Gene ID (NCBI) 9575
RRIDAB_2878497
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15516
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Circadian locomoter output cycles protein kaput (CLOCK), also named as BHLHE8 or KIAA0334, is a 846 amino acid protein, which contains one bHLH domain, one PAC domain, and two PAS domains. CLOCK localizes in the nucleus and cytoplasm. CLOCK) is expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. ARNTL/2-CLOCK heterodimers activate E-box element (5'-CACGTG-3') transcription of a number of proteins of the circadian clock, such as PER1 and PER2. The calculated molecular weight of CLOCK is 95kDa, we detected a 95-110 kDa protein by western blot. Our result is similar to the result of reseach paper (PMID: 30683868).

实验方案

Product Specific Protocols
WB protocol for CLOCK antibody 18094-1-APDownload protocol
IHC protocol for CLOCK antibody 18094-1-APDownload protocol
IF protocol for CLOCK antibody 18094-1-APDownload protocol
IP protocol for CLOCK antibody 18094-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

A druggable copper-signalling pathway that drives inflammation

Authors - Stéphanie Solier
WB

Sci Total Environ

Metastatic effects of perfluorooctanoic acid (PFOA) on Drosophila melanogaster with metabolic reprogramming and dysrhythmia in a multigenerational exposure scenario

Authors - Fangnon Firmin Fangninou
humanWB

Int J Mol Sci

Piperine Improves Lipid Dysregulation by Modulating Circadian Genes Bmal1 and Clock in HepG2 Cells.

Authors - Weiyun Zhang
  • KD Validated
mouseWB

J Agric Food Chem

3-MCPD Induced Mitochondrial Damage of Renal Cells Via the Rhythmic Protein BMAL1 Targeting SIRT3/SOD2

Authors - Shuang Guan
humanWB, IHC

Neoplasia

A long noncoding RNA perturbs the circadian rhythm of hepatoma cells to facilitate hepatocarcinogenesis.

Authors - Ming Cui
humanWB

J Agric Food Chem

Capsaicin Attenuates Oleic Acid-Induced Lipid Accumulation via the Regulation of Circadian Clock Genes in HepG2 Cells.

Authors - Run Li
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