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CLINT1 Polyclonal antibody

CLINT1 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 10470-1-AP

Print datasheet

Synonyms

clathrin interactor 1, CLINT, CLINT1, ENTH, Enthoprotin, EPN4, EPNR, Epsin 4, Epsin related protein, EPSINR, KIAA0171

验证数据展示

  • WB analysis of HeLa using 10470-1-AP

    WB analysis of HeLa using 10470-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10470-1-AP (CLINT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 10470-1-AP

    IHC staining of human breast cancer using 10470-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10470-1-AP (CLINT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 10470-1-AP

    IHC staining of human breast cancer using 10470-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10470-1-AP (CLINT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 10470-1-AP

    IF Staining of HepG2 using 10470-1-AP

    Immunofluorescent analysis of HepG2 cells, using CLINT1antibody 10470-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • FC experiment of HepG2 using 10470-1-AP

    FC experiment of HepG2 using 10470-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug CLINT1 antibody (10470-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • IP experiment of HeLa using 10470-1-AP

    IP experiment of HeLa using 10470-1-AP

    IP result of anti-CLINT1 (IP:10470-1-AP, 4ug; Detection:10470-1-AP 1:1000) with HeLa cells lysate 2800ug.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, mouse brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10470-1-AP targets CLINT1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, IHC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CLINT1 fusion protein Ag0754 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name clathrin interactor 1
Synonyms clathrin interactor 1, CLINT, CLINT1, ENTH, Enthoprotin, EPN4, EPNR, Epsin 4, Epsin related protein, EPSINR, KIAA0171
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 68 kDa, 80 kDa
GenBank Accession NumberBC004467
Gene Symbol CLINT1
Gene ID (NCBI) 9685
RRIDAB_2276405
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14677
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Clathrin interactor 1 (CLINT1), also known as enthoprotin or epsin-4, belongs to the epsin family of endocytic adapter proteins. CLINT1 interacts with clathrin, the adapter protein AP-1 and phosphoinositides. It may have a role in the formation of clathrin coated vesicles and trafficking between the trans-Golgi network and endosomes. Mutations in the gene of CLINT1 are associated with a susceptibility to schizophrenia and psychotic disorders. Calculated molecular weight of CLINT1 is 68 kDa. The migration of endogenous CLINT1 at 80 kDa has also been reported (PMID: 12213833; 15811338).

实验方案

Product Specific Protocols
WB protocol for CLINT1 antibody 10470-1-APDownload protocol
IHC protocol for CLINT1 antibody 10470-1-APDownload protocol
IF protocol for CLINT1 antibody 10470-1-APDownload protocol
IP protocol for CLINT1 antibody 10470-1-APDownload protocol
FC protocol for CLINT1 antibody 10470-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

BioE3 identifies specific substrates of ubiquitin E3 ligases

Authors - Orhi Barroso-Gomila
View Article