CLINT1 Polyclonal antibody

CLINT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10470-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

EPN4, Enthoprotin, ENTH, Clint, Clathrin-interacting protein localized in the trans-Golgi region

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, HeLa cells, Jurkat cells, mouse brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CLINT1 Ready-To-Use IHC Kit. CLINT1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

10470-1-AP targets CLINT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 CLINT1 fusion protein Ag0754 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 clathrin interactor 1
别名 EPN4, Enthoprotin, ENTH, Clint, Clathrin-interacting protein localized in the trans-Golgi region
计算分子量 68 kDa
观测分子量 68 kDa, 80 kDa
GenBank蛋白编号BC004467
基因名称 CLINT1
Gene ID (NCBI) 9685
RRIDAB_2276405
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14677
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Clathrin interactor 1 (CLINT1), also known as enthoprotin or epsin-4, belongs to the epsin family of endocytic adapter proteins. CLINT1 interacts with clathrin, the adapter protein AP-1 and phosphoinositides. It may have a role in the formation of clathrin coated vesicles and trafficking between the trans-Golgi network and endosomes. Mutations in the gene of CLINT1 are associated with a susceptibility to schizophrenia and psychotic disorders. Calculated molecular weight of CLINT1 is 68 kDa. The migration of endogenous CLINT1 at 80 kDa has also been reported (PMID: 12213833; 15811338).

实验方案

Product Specific Protocols
WB protocol for CLINT1 antibody 10470-1-APDownload protocol
IHC protocol for CLINT1 antibody 10470-1-APDownload protocol
IF protocol for CLINT1 antibody 10470-1-APDownload protocol
IP protocol for CLINT1 antibody 10470-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

BioE3 identifies specific substrates of ubiquitin E3 ligases

Authors - Orhi Barroso-Gomila
WB

bioRxiv

PKA Activity-Driven Modulation of Bidirectional Long-Distance transport of Lysosomal vesicles During Synapse Maintenance

Authors - Kerriann K Badal
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