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验证数据展示

  • WB analysis using 85831-4-RR

    WB analysis using 85831-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85831-4-RR (CLIC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 85831-4-RR

    WB analysis using 85831-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85831-4-RR (CLIC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat spleen using 85831-4-RR

    IHC staining of rat spleen using 85831-4-RR

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 85831-4-RR (CLIC1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CLIC1 antibody included
  • IHC staining of mouse kidney using 85831-4-RR

    IHC staining of mouse kidney using 85831-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85831-4-RR (CLIC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CLIC1 antibody included
  • IF Staining of HeLa using 85831-4-RR

    IF Staining of HeLa using 85831-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CLIC1 antibody (85831-4-RR, Clone: 250143E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85831-4-RR

    Affinity and Kinetic Characterization of 85831-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85831-4-RR against Human CLIC1 were performed. The affinity constant is 22.0 nM.

CLIC1 Recombinant monoclonal antibody

CLIC1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85831-4-RR

产品说明书

CloneNo. 250143E11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

G6, NCC27

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, rat spleen tissue, HL-60 cells, C2C12 cells, RAW 264.7 cells, C6 cells, mouse spleen tissue
Positive IHC detected inmouse kidney tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CLIC1 Ready-To-Use IHC Kit. CLIC1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85831-4-RR targets CLIC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6049

Product name: Recombinant human CLIC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-241 aa of BC064527

Sequence: MAEEQPQVELFVKAGSDGAKIGNCPFSQRLFMVLWLKGVTFNVTTVDTKRRTETVQKLCPGGQLPFLLYGTEVHTDTNKIEEFLEAVLCPPRYPKLAALNPESNTAGLDIFAKFSAYIKNSNPALNDNLEKGLLKALKVLDNYLTSPLPEEVDETSAEDEGVSQRKFLDGNELTLADCNLLPKLHIVQVVCKKYRGFTIPEAFRGVHRYLSNAYAREEFASTCPDDEEIELAYEQVAKALK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chloride intracellular channel 1
别名 G6, NCC27
计算分子量 27 kDa
观测分子量27-32 kDa
GenBank蛋白编号BC064527
基因名称 CLIC1
Gene ID (NCBI) 1192
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO00299
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chloride intracellular channel protein 1 (CLIC1) is a member of the chloride intracellular channel protein family. It plays a crucial role in various cellular processes, including the regulation of chloride ion transport, cell viability, and mitochondrial function. CLIC1 is involved in the formation of membrane-inserted channels that facilitate chloride ion movement, which is essential for maintaining cellular homeostasis and function (PMID: 32116799). Additionally, CLIC1 has been implicated in cancer progression, where it influences cell proliferation, migration, and invasion (PMID: 38027011).

实验方案

Product Specific Protocols
IF protocol for CLIC1 antibody 85831-4-RRDownload protocol
IHC protocol for CLIC1 antibody 85831-4-RRDownload protocol
WB protocol for CLIC1 antibody 85831-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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