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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 85831-4-RR (same clone as 85831-4-PBS)

    WB analysis using 85831-4-RR (same clone as 85831-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85831-4-RR (CLIC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85831-4-PBS in a different storage buffer formulation.

  • WB analysis using 85831-4-RR (same clone as 85831-4-PBS)

    WB analysis using 85831-4-RR (same clone as 85831-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85831-4-RR (CLIC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85831-4-PBS in a different storage buffer formulation.

  • IHC staining of rat spleen using 85831-4-RR (same clone as 85831-4-PBS)

    IHC staining of rat spleen using 85831-4-RR (same clone as 85831-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 85831-4-RR (CLIC1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85831-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CLIC1 antibody included
  • IHC staining of mouse kidney using 85831-4-RR (same clone as 85831-4-PBS)

    IHC staining of mouse kidney using 85831-4-RR (same clone as 85831-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85831-4-RR (CLIC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85831-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CLIC1 antibody included
  • IF Staining of HeLa using 85831-4-RR (same clone as 85831-4-PBS)

    IF Staining of HeLa using 85831-4-RR (same clone as 85831-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CLIC1 antibody (85831-4-RR, Clone: 250143E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85831-4-PBS in a different storage buffer formulation.

CLIC1 Recombinant antibody, PBS Only

CLIC1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85831-4-PBS

产品说明书

CloneNo. 250143E11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

G6, NCC27

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CLIC1 Ready-To-Use IHC Kit. CLIC1 primary antibody included.

产品信息

85831-4-PBS targets CLIC1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CLIC1 fusion protein Ag6049 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chloride intracellular channel 1
别名 G6, NCC27
计算分子量 27 kDa
观测分子量27-32 kDa
GenBank蛋白编号BC064527
基因名称 CLIC1
Gene ID (NCBI) 1192
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO00299
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Chloride intracellular channel protein 1 (CLIC1) is a member of the chloride intracellular channel protein family. It plays a crucial role in various cellular processes, including the regulation of chloride ion transport, cell viability, and mitochondrial function. CLIC1 is involved in the formation of membrane-inserted channels that facilitate chloride ion movement, which is essential for maintaining cellular homeostasis and function (PMID: 32116799). Additionally, CLIC1 has been implicated in cancer progression, where it influences cell proliferation, migration, and invasion (PMID: 38027011).