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CLIC1 Polyclonal antibody

CLIC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14545-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

G6, NCC27

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHL-60 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse colon tissue, human colon tissue, human pancreas tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Hela cells
Planning an IHC experiment? We recommend our IHCeasy CLIC1 Ready-To-Use IHC Kit. CLIC1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14545-1-AP targets CLIC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 CLIC1 fusion protein Ag6049 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chloride intracellular channel 1
别名 G6, NCC27
计算分子量 27 kDa
观测分子量 27-32 kDa
GenBank蛋白编号BC064527
基因名称 CLIC1
Gene ID (NCBI) 1192
RRIDAB_2081809
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00299
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CLIC1 antibody 14545-1-APDownload protocol
IHC protocol for CLIC1 antibody 14545-1-APDownload protocol
IF protocol for CLIC1 antibody 14545-1-APDownload protocol
IP protocol for CLIC1 antibody 14545-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Alzheimers Res Ther

Circular RNA APP contributes to Alzheimer's disease pathogenesis by modulating microglial polarization via miR-1906/CLIC1 axis

Authors - Deng-Pan Wu
ratIF

Oxid Med Cell Longev

Attenuation of ROS/Chloride Efflux-Mediated NLRP3 Inflammasome Activation Contributes to Alleviation of Diabetic Cardiomyopathy in Rats after Sleeve Gastrectomy.

Authors - Songhan Li
humanWB

Transl Oncol

Chloride intracellular channel 1 promotes esophageal squamous cell carcinoma proliferation via mTOR signalling

Authors - Huiwu Geng
  • KD Validated

Cell Biochem Biophys

CLIC1 Inhibition Protects Against Cellular Senescence and Endothelial Dysfunction Via the Nrf2/HO-1 Pathway.

Authors - Dezhao Lu
humanIHC

Anticancer Res

Chloride Intracellular Channel 1 Expression Is Associated With Poor Prognosis of Lung Adenocarcinoma.

Authors - Yuichiro Yasuda
mouseWB,IHC

Technol Cancer Res Treat

CLIC1 Drives Angiogenesis in Hepatocellular Carcinoma by Modulating VEGFA.

Authors - Xuyong Wei
  • KO Validated
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