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CLEC4G Polyclonal antibody

CLEC4G Polyclonal Antibody for IF-P, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF-P, IHC, ELISA

Conjugate

Unconjugated

Cat No : 18173-1-AP

Print datasheet

Synonyms

CLEC4G, LP2698, LSECtin, UNQ431

验证数据展示

  • IHC staining of human liver using 18173-1-AP

    IHC staining of human liver using 18173-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18173-1-AP (CLEC4G antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 18173-1-AP

    IHC staining of human liver using 18173-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18173-1-AP (CLEC4G antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human liver using 18173-1-AP

    IF Staining of human liver using 18173-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CLEC4G antibody (18173-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human liver using 18173-1-AP

    IF Staining of human liver using 18173-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CLEC4G antibody (18173-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inhuman liver tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver tissue

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18173-1-AP targets CLEC4G in IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF-P, IHC, ELISA
Tested Reactivity human
Immunogen CLEC4G fusion protein Ag12837 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name C-type lectin domain family 4, member G
Synonyms CLEC4G, LP2698, LSECtin, UNQ431
Calculated Molecular Weight 293 aa, 33 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC093691
Gene Symbol CLEC4G
Gene ID (NCBI) 339390
RRIDAB_2878512
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UXB4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CLEC4G, also known as LSECtin, is a member of the C-type lectin family of glycan-binding receptors that is expressed on sinusoidal endothelial cells in liver and lymph nodes. CLEC4G is a type II transmembrane protein of ∼40 kDa in size with a single C-type lectin-like domain at the COOH terminus, closest in homology to DC-SIGNR, DC-SIGN, and CD23. CLEC4G has been reported as a receptor for Japanese encephalitis virus (PubMed:24623090), ebolavirus (PubMed:16051304), SARS coronavirus/SARS-CoV (PubMed:16051304), lassa virus and Lymphocytic choriomeningitis virus glycoprotein (PubMed:22156524, PubMed:22673088).

实验方案

Product Specific Protocols
IHC protocol for CLEC4G antibody 18173-1-APDownload protocol
IF protocol for CLEC4G antibody 18173-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols