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验证数据展示

  • WB analysis of HeLa using 21013-1-AP

    WB analysis of HeLa using 21013-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21013-1-AP (CLEC18A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 21013-1-AP

    IHC staining of human kidney using 21013-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21013-1-AP (CLEC18A antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 21013-1-AP

    IHC staining of human kidney using 21013-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21013-1-AP (CLEC18A antibody) at dilution of 1:50 (under 40x lens).

CLEC18A Polyclonal antibody

CLEC18A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21013-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

MRLP2, MRCL1, MRCL, Mannose receptor-like protein 2, C-type lectin domain family 18 member A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21013-1-AP targets CLEC18A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CLEC18A fusion protein Ag15237 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 C-type lectin domain family 18, member A
别名 MRLP2, MRCL1, MRCL, Mannose receptor-like protein 2, C-type lectin domain family 18 member A
计算分子量 446 aa, 50 kDa
观测分子量 47-50 kDa
GenBank蛋白编号BC141808
基因名称 CLEC18A
Gene ID (NCBI) 348174
RRIDAB_10732842
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDA5D8T8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CLEC18A, or C-type lectin member 18A, is a protein that plays a significant role in the human immune system, particularly in the context of viral infections. CLEC18A is widely expressed, with the highest levels found in myeloid cells and the liver. It is a co-receptor for TLR3 (Toll-like receptor 3) and is involved in enhancing the host's immune response to viral infections, such as H5N1 influenza A virus (IAV). CLEC18A has been implicated in the development of mixed cryoglobulinemia (MC), an extrahepatic manifestation associated with hepatitis C virus (HCV). This antibody can recognize CLEC18A/B/C.

实验方案

Product Specific Protocols
WB protocol for CLEC18A antibody 21013-1-APDownload protocol
IHC protocol for CLEC18A antibody 21013-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols