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验证数据展示

  • WB analysis of BxPC-3 using 13590-1-AP

    WB analysis of BxPC-3 using 13590-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 13590-1-AP (CLEC10A/CD301 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

CLEC10A/CD301 Polyclonal antibody

CLEC10A/CD301 Polyclonal Antibody for WB, ELISA
Cat No. 13590-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

CLEC10A, CD301, C-type lectin superfamily member 14, C-type lectin domain family 10 member A, CLECSF14

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inBxPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13590-1-AP targets CLEC10A/CD301 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 CLEC10A/CD301 fusion protein Ag4490 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 C-type lectin domain family 10, member A
别名 CLEC10A, CD301, C-type lectin superfamily member 14, C-type lectin domain family 10 member A, CLECSF14
计算分子量 316 aa, 35 kDa
观测分子量 32 kDa, 28 kDa
GenBank蛋白编号BC039011
基因名称 CLEC10A
Gene ID (NCBI) 10462
RRIDAB_2083131
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IUN9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C-type lectin domain family 10 member A (CLEC10A), also known as CD301, macrophage galactose-type C-type lectin (MGL), DC-asialoglyco protein receptor (DC-ASGP-R) or human macrophage lectin (HML), is a type II transmembrane glycoprotein having a calcium-dependent carbohydrate recognition domain (PMID: 8598452; 11919201; 11698450). CLEC10A is expressed exclusively by myeloid professional antigen-presenting cells such as immature dendritic cells (DCs) and alternatively activated macrophages (PMID: 16998493). CLEC10A is a marker for human CD1c+ DCs (cDC2) (PMID: 29755453). It functions as an endocytic receptor for glycosylated antigens (PMID: 11919201).

实验方案

Product Specific Protocols
WB protocol for CLEC10A/CD301 antibody 13590-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols