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验证数据展示

  • IHC staining of mouse kidney using 82954-1-RR (same clone as 82954-1-PBS)

    IHC staining of mouse kidney using 82954-1-RR (same clone as 82954-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82954-1-RR (CLDN16 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82954-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 82954-1-RR (same clone as 82954-1-PBS)

    IHC staining of mouse kidney using 82954-1-RR (same clone as 82954-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82954-1-RR (CLDN16 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82954-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 82954-1-RR (same clone as 82954-1-PBS)

    IF Staining of HEK-293 using 82954-1-RR (same clone as 82954-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CLDN16 antibody (82954-1-RR, Clone: 230193G3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82954-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 82954-1-PBS

    IF Staining of HEK-293 using 82954-1-PBS

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CLDN16 antibody (82954-1-PBS, Clone: 230193G3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Claudin 16 Recombinant monoclonal antibody, PBS Only

Claudin 16 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 82954-1-PBS

产品说明书

CloneNo. 230193G3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, Indirect ELISA

别名

230193G3, Claudin-16, CLDN16, Paracellin-1, PCLN1

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82954-1-PBS targets Claudin 16 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag26531

Product name: Recombinant human CLDN16 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-73 aa of BC069682

Sequence: MTSRTPLLVTACLYYSYCNSRHLQQGVRKSKRPVFSHCQVPETQKTDTRHLSGARAGVCPCCHPDGLLATMRD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 claudin 16
别名 230193G3, Claudin-16, CLDN16, Paracellin-1, PCLN1
计算分子量 305 aa, 34 kDa
GenBank蛋白编号BC069682
基因名称 CLDN16
Gene ID (NCBI) 10686
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y5I7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. They have similar structures with four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-16 is primarily found in the kidneys and is responsible for regulating the reabsorption of magnesium and calcium ions. Mutations in the Claudin-16 gene can lead to a rare genetic disorder called familial hypomagnesemia with hypercalciuria and nephrocalcinosis (FHHNC).

实验方案

Product Specific Protocols
IF protocol for Claudin 16 antibody 82954-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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