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验证数据展示

  • WB analysis of A549 using 27638-1-AP

    WB analysis of A549 using 27638-1-AP

    A549 cells, HEK-293 cells, HepG2 cells, and MDCK cells were subjected to SDS PAGE followed by western blot with 27638-1-AP (Claudin 16 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 27638-1-AP

    WB analysis of HepG2 using 27638-1-AP

    WB result of Claudin 16 antibody (27638-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Claudin 16 transfected HepG2 cells.

  • Featured Product
  • KD/KO验证

Claudin 16 Polyclonal antibody

Claudin 16 Polyclonal Antibody for WB, ELISA
Cat No. 27638-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, canine

应用

WB, ELISA

别名

Claudin-16, CLDN16, Paracellin-1, PCLN1, PCLN-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, HEK-293 cells, MDCK cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27638-1-AP targets Claudin 16 in WB, ELISA applications and shows reactivity with human, canine samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, canine
免疫原

CatNo: Ag26531

Product name: Recombinant human CLDN16 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-73 aa of BC069682

Sequence: MTSRTPLLVTACLYYSYCNSRHLQQGVRKSKRPVFSHCQVPETQKTDTRHLSGARAGVCPCCHPDGLLATMRD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 claudin 16
别名 Claudin-16, CLDN16, Paracellin-1, PCLN1, PCLN-1
计算分子量 305 aa, 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC069682
基因名称 CLDN16
Gene ID (NCBI) 10686
RRIDAB_2880935
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5I7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Claudin 16 antibody 27638-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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