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验证数据展示

  • WB analysis using 87517-1-RR

    WB analysis using 87517-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87517-1-RR (CKMT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 87517-1-RR

    WB analysis using 87517-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87517-1-RR (CKMT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293T using 87517-1-RR

    WB analysis of HEK-293T using 87517-1-RR

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 87517-1-RR (CKMT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse heart using 87517-1-RR

    WB analysis of mouse heart using 87517-1-RR

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 87517-1-RR (CKMT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

CKMT2 Recombinant monoclonal antibody

CKMT2 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 87517-1-RR

产品说明书

CloneNo. 252920F5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Basic-type mitochondrial creatine kinase, CKMT 2, Creatine kinase S-type, mitochondrial, EC:2.7.3.2, Mib CK

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat heart tissue, HEK-293T cells, mouse heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, human heart tissue
Planning an IHC experiment? We recommend our IHCeasy® CKMT2 Ready-To-Use IHC Kit. CKMT2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87517-1-RR targets CKMT2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3971

Product name: Recombinant human CKMT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 57-419 aa of BC029140

Sequence: LRKHNNCMAECLTPAIYAKLRNKVTPNGYTLDQCIQTGVDNPGHPFIKTVGMVAGDEESYEVFADLFDPVIKLRHNGYDPRVMKHTTDLDASKITQGQFDEHYVLSSRVRTGRSIRGLSLPPACTRAERREVENVAITALEGLKGDLAGRYYKLSEMTEQDQQRLIDDHFLFDKPVSPLLTCAGMARDWPDARGIWHNYDKTFLIWINEEDHTRVISMEKGGNMKRVFERFCRGLKEVERLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHVRIPKLSKDPRFSKILENLRLQKRGTGGVDTAAVADVYDISNIDRIGRSEVELVQIVIDGVNYLVDCEKKLERGQDIKVPPPLPQFGKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 creatine kinase, mitochondrial 2 (sarcomeric)
别名 Basic-type mitochondrial creatine kinase, CKMT 2, Creatine kinase S-type, mitochondrial, EC:2.7.3.2, Mib CK
计算分子量 419 aa, 48 kDa
观测分子量 41-48 kDa
GenBank蛋白编号BC029140
基因名称 CKMT2
Gene ID (NCBI) 1160
RRIDAB_3745594
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP17540
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CKMT2 (Creatine kinase S-type, mitochondrial), also known as s-MtCK, is one of the creatine kinase (CK) isozymes that are tightly coupled to adenosine triphosphate (ATP) output via adenine nucleotide transport proteins or carriers, making it an important player in ATP synthesis and respiratory chain activity (PMID: 38172162). Enhancing Ckmt2 expression or activity can protect against myocardial injury caused by ischemia-reperfusion injury (PMID: 39457679).

实验方案

Product Specific Protocols
WB protocol for CKMT2 antibody 87517-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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