• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84485-5-RR (same clone as 84485-5-PBS)

    WB analysis using 84485-5-RR (same clone as 84485-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84485-5-RR (CKAP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84485-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 84485-5-RR (same clone as 84485-5-PBS)

    IHC staining of mouse brain using 84485-5-RR (same clone as 84485-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84485-5-RR (CKAP4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84485-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CKAP4 antibody included
  • IHC staining of mouse brain using 84485-5-RR (same clone as 84485-5-PBS)

    IHC staining of mouse brain using 84485-5-RR (same clone as 84485-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84485-5-RR (CKAP4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84485-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CKAP4 antibody included
  • Affinity and Kinetic Characterization of 84485-5-RR

    Affinity and Kinetic Characterization of 84485-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84485-5-RR against Human CKAP4 were performed. The affinity constant is 15.1 nM.

    {{ptg:ProductFeaturesInfo}}

CKAP4 Recombinant monoclonal antibody, PBS Only

CKAP4 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84485-5-PBS

产品说明书

CloneNo. 241871C5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

241871C5, 63 kDa membrane protein, CLIMP 63, Climp-63, ERGIC 63

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy CKAP4 Ready-To-Use IHC Kit. CKAP4 primary antibody included.

产品信息

84485-5-PBS targets CKAP4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag10022

Product name: Recombinant human CKAP4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 254-602 aa of BC082972

Sequence: IFTEVQKRSQKEINDMKAKVASLEESEGNKQDLKALKEAVKEIQTSAKSREWDMEALRSTLQTMESDIYTEVRELVSLKQEQQAFKEAADTERLALQALTEKLLRSEESVSRLPEEIRRLEEELRQLKSDSHGPKEDGGFRHSEAFEALQQKSQGLDSRLQHVEDGVLSMQVASARQTESLESLLSKSQEHEQRLAALQGRLEGLGSSEADQDGLASTVRSLGETQLVLYGDVEELKRSVGELPSTVESLQKVQEQVHTLLSQDQAQAARLPPQDFLDRLSSLDNLKASVSQVEADLKMLRTAVDSLVAYSVKIETNENNLESAKGLLDDLRNDLDRLFVKVEKIHEKV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytoskeleton-associated protein 4
别名 241871C5, 63 kDa membrane protein, CLIMP 63, Climp-63, ERGIC 63
计算分子量 602 aa, 66 kDa
观测分子量 63 kDa
GenBank蛋白编号BC082972
基因名称 CKAP4
Gene ID (NCBI) 10970
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ07065
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CKAP4 (Cytoskeleton-associated protein 4), also known as CLIMP-63 (cytoskeleton-linking membrane protein 63), is a 63 kDa cytoskeleton-linking membrane protein. CKAP4 is a novel DKK1-binding protein on the cell surface membrane of Madin-Darby canine kidney (MDCK) epithelial cells, and evaluated CKAP4-mediated DKK1 signaling in cancer cell proliferation (PMID: 27322059). In addition, CKAP4 was modified with palmitate at Cys100 by palmitoyl acyltransferase DHHC2 (PMID: 18296695), and palmitoylation was required for CKAP4 localization to PM lipid rafts to promote cancer cell proliferation (PMID: 31744930).