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验证数据展示

  • WB analysis using 84485-5-RR (same clone as 84485-5-PBS)

    WB analysis using 84485-5-RR (same clone as 84485-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84485-5-RR (CKAP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84485-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 84485-5-RR (same clone as 84485-5-PBS)

    IHC staining of mouse brain using 84485-5-RR (same clone as 84485-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84485-5-RR (CKAP4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84485-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CKAP4 antibody included
  • IHC staining of mouse brain using 84485-5-RR (same clone as 84485-5-PBS)

    IHC staining of mouse brain using 84485-5-RR (same clone as 84485-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84485-5-RR (CKAP4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84485-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CKAP4 antibody included
  • Affinity and Kinetic Characterization of 84485-5-RR

    Affinity and Kinetic Characterization of 84485-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84485-5-RR against Human CKAP4 were performed. The affinity constant is 15.1 nM.

CKAP4 Recombinant antibody, PBS Only

CKAP4 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84485-5-PBS

产品说明书

CloneNo. 241871C5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

241871C5, 63 kDa membrane protein, CLIMP 63, Climp-63, ERGIC 63

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CKAP4 Ready-To-Use IHC Kit. CKAP4 primary antibody included.

产品信息

84485-5-PBS targets CKAP4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 CKAP4 fusion protein Ag10022 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytoskeleton-associated protein 4
别名 241871C5, 63 kDa membrane protein, CLIMP 63, Climp-63, ERGIC 63
计算分子量 602 aa, 66 kDa
观测分子量 63 kDa
GenBank蛋白编号BC082972
基因名称 CKAP4
Gene ID (NCBI) 10970
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ07065
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CKAP4 (Cytoskeleton-associated protein 4), also known as CLIMP-63 (cytoskeleton-linking membrane protein 63), is a 63 kDa cytoskeleton-linking membrane protein. CKAP4 is a novel DKK1-binding protein on the cell surface membrane of Madin-Darby canine kidney (MDCK) epithelial cells, and evaluated CKAP4-mediated DKK1 signaling in cancer cell proliferation (PMID: 27322059). In addition, CKAP4 was modified with palmitate at Cys100 by palmitoyl acyltransferase DHHC2 (PMID: 18296695), and palmitoylation was required for CKAP4 localization to PM lipid rafts to promote cancer cell proliferation (PMID: 31744930).