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验证数据展示

  • WB analysis using 86689-1-RR (same clone as 86689-1-PBS)

    WB analysis using 86689-1-RR (same clone as 86689-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86689-1-RR (CKAP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86689-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86689-1-RR (same clone as 86689-1-PBS)

    IF Staining of HeLa using 86689-1-RR (same clone as 86689-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CKAP2 antibody (86689-1-RR, Clone: 251698F2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86689-1-PBS in a different storage buffer formulation.

CKAP2 Recombinant monoclonal antibody, PBS Only

CKAP2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86689-1-PBS

产品说明书

CloneNo. 251698F2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

Cytoskeleton-associated protein 2, LB1, se20-10, TMAP, Tumor- and microtubule-associated protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86689-1-PBS targets CKAP2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag21901

Product name: Recombinant human CKAP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-356 aa of BC136332

Sequence: MSTPAVPQDLQLPPSQRAQSAFKEQRRQKLKEHLLRRKTLFAYKQENEMLSSRDQRVVTSEDQVQEGTKVLKLKTKMADKENMKRPAESKNNTVVGKHCIPLKPSNELTNSTVVIDTHKPKDSNQTPHLLLTEDDPQSQHMTLSQAFHLKNNSKKKQMTTEKQKQDANMPKKPVLGSYRGQIVQSKINSFRKPLQVKDESSAATKKLSATIPKATKPQPVNTSSVTVKSNRSSNMTATTKFVSTTSQNTQLVRPPIRSHHSNTRDTVKQGISRTSANVTIRKGPHEKELLQSKTALSSVKTSSSQGIIRNKTLSRSIASEVVARPASLSNDKLMEKSEPVDQRRHTAGKAIVDSRS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytoskeleton associated protein 2
别名 Cytoskeleton-associated protein 2, LB1, se20-10, TMAP, Tumor- and microtubule-associated protein
计算分子量 683 aa, 77 kDa
观测分子量 75-85 kDa
GenBank蛋白编号BC136332
基因名称 CKAP2
Gene ID (NCBI) 26586
RRIDAB_3745054
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8WWK9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cytoskeleton-associated protein 2 (CKAP2), also known as tumor-associated microtubule-associated protein (TMAP), is implicated in the regulation of cell proliferation (PMID: 30119212). CKAP2 is a MAP expressed during mitosis that localizes to centrosomes and spindles before being targeted for degradation by the anaphase-promoting complex/cyclosome at mitotic exit (PMID: 38381793, 17339342).

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