验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse heart tissue, mouse liver tissue, mouse pancreas tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32928-1-AP targets CIR in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag38647 Product name: Recombinant human CIR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-160 aa of NM_004882.3 Sequence: MGKSFANFMCKKDFHPASKSNIKKVWMAEQKISYDKKKQEELMQQYLKEQESYDNRLLMGDERVKNGLNFMYEAPPGAKKENKEKEETEGETEYKFEWQKGAPREKYAKDDMNIRDQPFGIQVRNVRCIKCHKWGHVNTDRECPLFGLSGINASSVPTDG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CBF1 interacting corepressor |
| 别名 | CBF1-interacting corepressor, CIR1, Corepressor interacting with RBPJ 1, Recepin |
| 计算分子量 | 52kDa，450aa |
| 观测分子量 | 23 kDa |
| GenBank蛋白编号 | NM_004882.3 |
| 基因名称 | CIR |
| Gene ID (NCBI) | 9541 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q86X95 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CIR, a protein originally identified as a CBF1-interacting protein and reported to act as a transcriptional corepressor. CIR is a member of the family of SR-related proteins and that CIR plays a role in splicing regulation. In addition to a basic lysine and acidic serine-rich (BA) domain and a zinc knuckle-like motif, CIR has an arginine/serine dipeptide repeat (RS) domain in its C terminal region. The RS domain has been reported to be present in the superfamily of SR proteins, which are involved in splicing reactions (PMID: 15652350).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CIR antibody 32928-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
