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验证数据展示

  • WB analysis using 11813-1-AP (same clone as 11813-1-PBS)

    WB analysis using 11813-1-AP (same clone as 11813-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11813-1-AP (CILP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11813-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 11813-1-AP (same clone as 11813-1-PBS)

    IF Staining of HEK-293 using 11813-1-AP (same clone as 11813-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 11813-1-AP (CILP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11813-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 11813-1-AP (same clone as 11813-1-PBS)

    IF Staining of HEK-293 using 11813-1-AP (same clone as 11813-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CILP2 antibody (11813-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11813-1-PBS in a different storage buffer formulation.

CILP2 Polyclonal antibody, PBS Only

CILP2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 11813-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

CILP-2, Cartilage intermediate layer protein 2 C2, Cartilage intermediate layer protein 2 C1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

11813-1-PBS targets CILP2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag2366

Product name: Recombinant human CILP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 304-653 aa of BC034926

Sequence: ALVTATLGGEELEPAPSLPRPLPATVGVTQPYLDRLGYRRTDHDDPAFKRNGFRINLAKPRPGDPAEANGPVYPWRSLRECQGAPVTASHFRFARVEADKYEYNVVPFREGTPASWTGDLLAWWPNPQEFRACFLKVKIQGPQEYMVRSHNAGGSHPRTRGQLYGLRDARSVRDPERPGTSAACVEFKCSGMLFDQRQVDRTLVTIMPQGSCRRVAVNGLLRDYLTRHPPPVPAEDPAAFSMLAPLDPLGHNYGVYTVTDQSPRLAKEIAIGRCFDGSSDGFSREMKADAGTAVTFQCREPPAGRPSLFQRLLESPATALGDIRREMSEAAQAQARASGPLRTRRGRVRQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cartilage intermediate layer protein 2
别名 CILP-2, Cartilage intermediate layer protein 2 C2, Cartilage intermediate layer protein 2 C1
计算分子量 126 kDa
观测分子量 90 kDa
GenBank蛋白编号BC034926
基因名称 CILP2
Gene ID (NCBI) 148113
RRIDAB_2877795
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IUL8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CILP2, also named as Cartilage intermediate layer protein 2, is a 1156 amino acid protein, which is expressed in articular chondrocytes but not in knee meniscal cartilage cells. CILP2 localizes to the intermediate to deep zone of articular cartilage. CILP2 may play a role in cartilage scaffolding. Given the full-length molecular mass of CILP2 is 125 kDa, but the most CILP2 protein in articular cartilage is cleaved,mostly likely at the furin cleavage site. CILP2 protein is detected as a major band on SDS-page migrating at about 90 kDa. (PMID: 21880736)

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