CILP2 Polyclonal antibody
CILP2 Polyclonal Antibody for WB,IF/ICC,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB,IF/ICC,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, K-562 cells, SW480 cells, mouse brain tissue |
Positive IF detected in | HEK-293 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
11813-1-AP targets CILP2 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB,IF/ICC,ELISA |
Cited Applications | WB |
Tested Reactivity | human, mouse |
Cited Reactivity | mouse |
Immunogen | CILP2 fusion protein Ag2366 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | cartilage intermediate layer protein 2 |
Synonyms | CILP 2, CILP2, CLIP 2 |
Calculated Molecular Weight | 126 kDa |
Observed Molecular Weight | 90 kDa |
GenBank Accession Number | BC034926 |
Gene Symbol | CILP2 |
Gene ID (NCBI) | 148113 |
RRID | AB_2877795 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q8IUL8 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CILP2, also named as Cartilage intermediate layer protein 2, is a 1156 amino acid protein, which is expressed in articular chondrocytes but not in knee meniscal cartilage cells. CILP2 localizes to the intermediate to deep zone of articular cartilage. CILP2 may play a role in cartilage scaffolding. Given the full-length molecular mass of CILP2 is 125 kDa, but the most CILP2 protein in articular cartilage is cleaved,mostly likely at the furin cleavage site. CILP2 protein is detected as a major band on SDS-page migrating at about 90 kDa. (PMID: 21880736)
实验方案
Product Specific Protocols | |
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WB protocol for CILP2 antibody 11813-1-AP | Download protocol |
IF protocol for CILP2 antibody 11813-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Rep Endothelial cells regulated by RNF20 orchestrate the proliferation and differentiation of neural precursor cells during embryonic development |