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验证数据展示

  • WB analysis of mouse skin using 12247-1-AP

    WB analysis of mouse skin using 12247-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 12247-1-AP (CHURC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 12247-1-AP

    IHC staining of human brain using 12247-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12247-1-AP (CHURC1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 12247-1-AP

    IHC staining of human brain using 12247-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12247-1-AP (CHURC1 Antibody) at dilution of 1:50 (under 40x lens).

CHURC1 Polyclonal antibody

CHURC1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12247-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

C14orf52, chch, CHURC1, churchill domain containing 1, My015, Protein Churchill

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse skin tissue, HeLa cells, mouse liver tissue
Positive IHC detected inhuman brain tissue, human gliomas tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12247-1-AP targets CHURC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CHURC1 fusion protein Ag2892 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 churchill domain containing 1
别名 C14orf52, chch, CHURC1, churchill domain containing 1, My015, Protein Churchill
计算分子量 13 kDa
观测分子量 13 kDa
GenBank蛋白编号BC020550
基因名称 CHURC1
Gene ID (NCBI) 91612
RRIDAB_10666204
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WUH1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHURC1 (churchill domain containing 1), also known as C14orf52 or chch. The function of CHURC1 has not been widely studied, and is yet to be fully elucidated. It may be a transcriptional activator that mediates FGF signaling during neural development.The MW of this protein is 13 kDa, and Catalog#12247-1-AP specially recognises the 13 kDa protein.

实验方案

Product Specific Protocols
WB protocol for CHURC1 antibody 12247-1-APDownload protocol
IHC protocol for CHURC1 antibody 12247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols