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验证数据展示

  • WB analysis of HT-29 using 66623-1-Ig (same clone as 66623-1-PBS)

    WB analysis of HT-29 using 66623-1-Ig (same clone as 66623-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 66623-1-Ig (CHST4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66623-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66623-1-Ig (same clone as 66623-1-PBS)

    IHC staining of human lung cancer using 66623-1-Ig (same clone as 66623-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66623-1-Ig (CHST4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66623-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66623-1-Ig (same clone as 66623-1-PBS)

    IF Staining of HeLa using 66623-1-Ig (same clone as 66623-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CHST4 antibody (66623-1-Ig, Clone: 1E1A4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66623-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CHST4 Monoclonal antibody, PBS Only

CHST4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66623-1-PBS

产品说明书

CloneNo. 1E1A4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Gn6st-2, Gn6st 2, GlcNAc6ST-2, GlcNAc6ST 2, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66623-1-PBS targets CHST4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3987

Product name: Recombinant human CHST4 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 177-386 aa of BC035282

Sequence: DPSLNLHIVHLVRDPRAVFRSRERTKGDLMIDSRIVMGQHEQKLKKEDQPYYVMQVICQSQLEIYKTIQSLPKALQERYLLVRYEDLARAPVAQTSRMYEFVGLEFLPHLQTWVHNITRGKGMGDHAFHTNARDALNVSQAWRWSLPYEKVSRLQKACGDAMNLLGYRHVRSEQEQRNLLLDLLSTWTVPEQIH

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4
别名 Gn6st-2, Gn6st 2, GlcNAc6ST-2, GlcNAc6ST 2, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3
计算分子量 370 aa, 43 kDa
观测分子量 48 kDa
GenBank蛋白编号BC035282
基因名称 CHST4
Gene ID (NCBI) 10164
RRIDAB_2881983
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ8NCG5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHST4, also named as HEC GlcNAc6ST and GlcNAc6ST 2, is one of a major GlcNAc-6-O-sulfotransferases involved in synthesizing the L-selectin ligands mediating lymphocyte homing. CHST4 is associated with the expression of MECA-79 epitope on HEV. It has been reported that the transcripts of CHST4 can be detected in lymph node, liver, pancreas and high endothelial venules (HEVs) but at a low level in HUVECs. Besides, CHST4 is expressed at a high level in gastric and colorectal cancer patients compared with normal mucosa. (PMID:30093410, 27748735, 10330415)