验证数据展示
经过测试的应用
Positive WB detected in | mouse liver tissue |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human kidney tissue, human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | Hela cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20141-1-AP targets CHST13-Specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | carbohydrate (chondroitin 4) sulfotransferase 13 |
别名 | CHST13, Chondroitin 4-sulfotransferase 3, Chondroitin 4-O-sulfotransferase 3, C4ST-3, C4ST3 |
计算分子量 | 39 kDa |
观测分子量 | 25 kDa |
GenBank蛋白编号 | NM_152889 |
基因名称 | CHST13 |
Gene ID (NCBI) | 166012 |
RRID | AB_10667458 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q8NET6 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CHST13, also named as C4ST3, belongs to the sulfotransferase 2 family. CHST13 catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. CHST13 transfers sulfate to the C4 hydroxyl of beta1,4-linked GalNAc that is substituted with a beta-linked glucuronic acid at the C-3 hydroxyl. No activity toward dermatan. The antibody is specific to CHST13.
实验方案
Product Specific Protocols | |
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WB protocol for CHST13-Specific antibody 20141-1-AP | Download protocol |
IHC protocol for CHST13-Specific antibody 20141-1-AP | Download protocol |
IF protocol for CHST13-Specific antibody 20141-1-AP | Download protocol |
IP protocol for CHST13-Specific antibody 20141-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |