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验证数据展示

  • WB analysis using 12126-2-AP

    WB analysis using 12126-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12126-2-AP (CHRAC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 12126-2-AP

    IHC staining of mouse lung using 12126-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12126-2-AP (CHRAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CHRAC1 Polyclonal antibody

CHRAC1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12126-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CHARC1, CHARC15, CHRAC 1, CHRAC 15, CHRAC-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, K-562 cells
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12126-2-AP targets CHRAC1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2770

Product name: Recombinant human CHRAC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-131 aa of BC015891

Sequence: MADVVVGKDKGGEQRLISLPLSRIRVIMKSSPEVSSINQEALVLTAKATELFVQCLATYSYRHGSGKEKKVLTYSDLANTAQQSETFQFLADILPKKILASKYLKMLKEEKREEDEENDNDNESDHDEADS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromatin accessibility complex 1
别名 CHARC1, CHARC15, CHRAC 1, CHRAC 15, CHRAC-1
计算分子量 131 aa, 15 kDa
观测分子量 15 kDa
GenBank蛋白编号BC015891
基因名称 CHRAC1
Gene ID (NCBI) 54108
RRIDAB_2291729
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRG0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHRAC1 antibody 12126-2-APDownload protocol
IHC protocol for CHRAC1 antibody 12126-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols