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CHMP7 Monoclonal antibody

CHMP7 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F8F6

Cat No : 68406-1-Ig

Print datasheet

Synonyms

CHMP family, member 7, CHMP7, Chromatin modifying protein 7

验证数据展示

  • WB analysis using 68406-1-Ig

    WB analysis using 68406-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68406-1-Ig (CHMP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IHC staining of human prostate cancer using 68406-1-Ig

    IHC staining of human prostate cancer using 68406-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68406-1-Ig (CHMP7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 68406-1-Ig

    IHC staining of human prostate cancer using 68406-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 68406-1-Ig (CHMP7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 68406-1-Ig

    IF Staining of A431 using 68406-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CHMP7 antibody (68406-1-Ig, Clone: 2F8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, MCF-7 cells, HEK-293 cells, HepG2 cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68406-1-Ig targets CHMP7 in WB, IF, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, rat
Immunogen CHMP7 fusion protein Ag9641 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CHMP family, member 7
Synonyms CHMP family, member 7, CHMP7, Chromatin modifying protein 7
Calculated Molecular Weight 453 aa, 51 kDa
Observed Molecular Weight 51-55 kDa
GenBank Accession NumberBC019110
Gene Symbol CHMP7
Gene ID (NCBI) 91782
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8WUX9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CHMP7 antibody 68406-1-IgDownload protocol
IHC protocol for CHMP7 antibody 68406-1-IgDownload protocol
IF protocol for CHMP7 antibody 68406-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols